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pnp elisa kit :: Human paraneoplastic pemphigus (PNP) antibody ELISA Kit

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Catalog # MBS704248
Unit / Price DISCONTINUED
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pnp elisa kit
Product Name

paraneoplastic pemphigus (PNP) antibody, ELISA Kit

Also Known As

Human paraneoplastic pemphigus, PNP antibody ELISA Kit

Product Synonym Names
Human paraneoplastic pemphigus (PNP) antibody ELISA kit; paraneoplastic pemphigus (PNP) antibody
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
913
3D Structure
ModBase 3D Structure for E4R2X1
Species Reactivity
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of pnp elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forpnppurchase
MBS704248 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the paraneoplastic pemphigus (PNP) antibody, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing pnp. The ELISA analytical biochemical technique of the MBS704248 kit is based on pnp antibody-pnp antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect pnp antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, pnp. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
pnp elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated withPNP antigen. Standards or samples are then added to theappropriate microtiter plate wells with biotin-conjugated PNP andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain Paraneoplastic pemphigus(PNP)antibody, biotin-conjugated PNP and enzyme-conjugated Avidinwill exhibit a change in color. The enzyme-substrate reaction isterminated by the addition of a sulphuric acid solution and thecolor change is measured spectrophotometrically at awavelength of 450 nm +/- 2 nm. The concentration ofParaneoplastic pemphigus(PNP)antibody in the samples is thendetermined by comparing the O.D. of the samples to thestandard curve.
Sample Manual Insert of MBS704248. Click to request current manual
NCBI/Uniprot data below describe general gene information for pnp. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
101,876 Da[Similar Products]
NCBI Official Full Name
PnP
NCBI Official Symbol
NCBI Protein Information
pnp
UniProt Protein Name
Polyribonucleotide nucleotidyltransferase
UniProt Synonym Protein Names
Polynucleotide phosphorylase
UniProt Gene Name
UniProt Synonym Gene Names
BBMN68_1576  [Similar Products]
UniProt Entry Name
E4R2X1_BIFLM
UniProt Comments for pnp
Function: Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction UniRule annotationHAMAP-Rule:MF_01595

Catalytic activity: RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotationHAMAP-Rule:MF_01595

Cofactor: Magnesium UniRule annotationHAMAP-Rule:MF_01595
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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