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Human Phospholipid Screen IgG/IgM ELISA Kit

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Catalog # MBS494699-ORG 529
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Phospholipid Screen IgG/IgM elisa kit
Product Name

Phospholipid Screen IgG/IgM, ELISA Kit

Popular Item
Also Known As

Anti-Phospholipid Screen IgG/IgM ELISA

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
The microplate is coated with a mixture of highly purified cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and human beta-2-glycoprotein I. Special coating processes, developed by the manufacturer guarantee for the native immunogenic structure of the phospholipids after immobilisation on the solid phase. The Anti- Phospholipid Screen test kit is specific for autoantibodies directed against phospholipid, beta-2-glycoproteinI or the complex of negatively-charged phospholipids and beta-2- glycoproteinI. No cross reactivity was observed to anti-DNA antibodies and those types of antibodies occurring in syphilis.
Samples
Human serum or plasma
Sensitivity
The lower detection limits for Anti-Phospholipid Screen IgG and IgM were determined at 0.5 U/ml.
Intended Uses
The ORGENTEC Anti-Phospholipid Screen IgG/IgM assay is an enzyme immunoassay (EIA) intended to screen for the presence of IgG and IgM class autoantibodies against cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and beta-2- glycoprotein I in human serum or plasma. It is for research use only, not for use in diagnostic procedures.
Preparation and Storage
2 - 8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Phospholipid Screen IgG/IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPhospholipid Screen IgG/IgMpurchase
MBS494699-ORG 529 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospholipid Screen IgG/IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Phospholipid Screen IgG/IgM. The ELISA analytical biochemical technique of the MBS494699-ORG 529 kit is based on Phospholipid Screen IgG/IgM antibody-Phospholipid Screen IgG/IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Phospholipid Screen IgG/IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Phospholipid Screen IgG/IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Phospholipid Screen IgG/IgM elisa kit
Principle of the assay: A mixture of highly purified cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and human beta-2-glycoprotein I is bound to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG or IgM immunologically detects the bound antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end product. The intensity of this yellow color is measured photometrically at 450 nm.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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