View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Human Phospholipid Screen IgG/IgM ELISA Kit Catalog # MBS494699-ORG 529 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Phospholipid Screen IgG/IgM, ELISA Kit Popular Item Full Product Name Anti-Phospholipid Screen IgG/IgM ELISA Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Request for Current Manual Insert Request Current Manual Species Reactivity Human Specificity The microplate is coated with a mixture of highly purified cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and human beta-2-glycoprotein I. Special coating processes, developed by the manufacturer guarantee for the native immunogenic structure of the phospholipids after immobilisation on the solid phase. The Anti- Phospholipid Screen test kit is specific for autoantibodies directed against phospholipid, beta-2-glycoproteinI or the complex of negatively-charged phospholipids and beta-2- glycoproteinI. No cross reactivity was observed to anti-DNA antibodies and those types of antibodies occurring in syphilis. Samples Human serum or plasma Sensitivity The lower detection limits for Anti-Phospholipid Screen IgG and IgM were determined at 0.5 U/ml. Intended Uses The ORGENTEC Anti-Phospholipid Screen IgG/IgM assay is an enzyme immunoassay (EIA) intended to screen for the presence of IgG and IgM class autoantibodies against cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and beta-2- glycoprotein I in human serum or plasma. It is for research use only. Preparation and Storage 2 - 8 degree C Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Phospholipid Screen IgG/IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for Phospholipid Screen IgG/IgM purchase MBS494699-ORG 529 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospholipid Screen IgG/IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Phospholipid Screen IgG/IgM. The ELISA analytical biochemical technique of the MBS494699-ORG 529 kit is based on Phospholipid Screen IgG/IgM antibody-Phospholipid Screen IgG/IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Phospholipid Screen IgG/IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Phospholipid Screen IgG/IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for Phospholipid Screen IgG/IgM elisa kit Principle of the assay: A mixture of highly purified cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and human beta-2-glycoprotein I is bound to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG or IgM immunologically detects the bound antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end product. The intensity of this yellow color is measured photometrically at 450 nm. Product Categories/Family for Phospholipid Screen IgG/IgM elisa kit Autoimmune; Immunology; Research Assays; Assay Categories Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP