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Human Tryptophan ELISA Kit

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Catalog # MBS495079
Unit / Price
  96 Tests  /  $835 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Tryptophan, ELISA Kit

Popular Item
Also Known As

Tryptophan ELISA, 96 well

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Samples
urine, serum and plasma samples
Preparation and Storage
2 - 8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Tryptophan elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTryptophanpurchase
MBS495079 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tryptophan, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Tryptophan. The ELISA analytical biochemical technique of the MBS495079 kit is based on Tryptophan antibody-Tryptophan antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Tryptophan antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Tryptophan. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Tryptophan elisa kit
Intended Uses: Enzyme Immunoassay for the quantitative determination of Tryptophan in urine, serum and plasma samples.

Principle of the Assay: After extraction and derivatization Tryptophan is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.

Background: L-Tryptophan is one of the essential amino acids for the human metabolism and must be part of its diet. In humans it serves as precursor for the synthesis of the neurotransmitters serotonin and tryptamine as well as for the synthesis of nicotinic acid and the epiphyseal hormone melatonin. Tryptophan is catabolized to kynurenine through the enzyme IDO (indoleamine-2,3-dioxygenase). Increased IDO activity is an expression of neuro-endocrine-immunological dysregulation, which is often associated with depressive symptoms such as bipolar disorder (manic depression). In addition Tryptophan and its metabolites regulate neurobehavioral effects such as appetite, sleeping-waking-rhythm and pain perception.

Typical Testing Data/Standard Curve (for reference only) of Tryptophan elisa kit
Tryptophan elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS495079. Click to request current manual
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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