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Human hsa-mir-205 Real-time RT-PCR PCR Kit

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Catalog # MBS826104
Unit / Price
  50 rxn  /  $300 +1 FREE 8GB USB
  100 rxn  /  $425 +1 FREE 8GB USB
  200 rxn  /  $615 +1 FREE 8GB USB
Product Name

hsa-mir-205 Real-time RT-PCR, PCR Kit

Also Known As

hsa-mir-205 Real-time RT-PCR Detection Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Samples
miRNA, Total RNA
PCR Assay Type
2-Step qRT-PCR
Detection Range
Seven orders of magnitude
Sensitivity
10 copies of a standard mimics and as little as 25 pg of total RNA
Platform
ABI 7500 System/ViiA 7 System/StepOne/StepOnePlus/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
Components
50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.
100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.
200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for 6 months.
Product Note
Our PCR Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of hsa-mir-205 Real-time RT-PCR pcr kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
hsa-mir-205 Real-time RT-PCR pcr kit
Detect mature miRNA hsa-mir-205 expression.
Applications Tested/Suitable for hsa-mir-205 Real-time RT-PCR pcr kit
Real-time RT-PCR
The miRNAs Real-Time RT-PCR Detection Kit contains the whole reagent including the stem-loop RT primer and a pair of specific PCR primer set. It provides a sensitive and specific method of real-time PCR for quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.

Synthesized standard amplifiction curve (10nM/100pM)
hsa-mir-205 Real-time RT-PCR pcr kit Amplication image
Amplifiction curve in total RNA from 293T/A549 cells
hsa-mir-205 Real-time RT-PCR pcr kit Amplication #2 image
Dissociation curve (Note: the minor peak on the left is the negative control)
hsa-mir-205 Real-time RT-PCR pcr kit Dissociation Curve image
Electrophoresis Image (20bp marker)
hsa-mir-205 Real-time RT-PCR pcr kit Electrophoresis image
Sample Manual Insert of MBS826104. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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