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COL5A2 purified protein :: COLLAGEN V Purified Protein

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Catalog # MBS230111
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COL5A2 purified protein
Product Name

COLLAGEN V (COL5A2), Purified Protein

Full Product Name

NATIVE HUMAN COLLAGEN V

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (189896641..190044605, complement). Location: 2q14-q32
OMIM
120190
3D Structure
ModBase 3D Structure for P05997
Species Reactivity
Human
Specificity
COLLAGEN V
Purity/Purification
SDS PAGE: >95%

Contaminants:
Form/Format
Purified (Purified protein from human placenta - lyophilised)
Preparation
Purified by acetic acid extraction after pepsin treatment followed by serial salt precipitations and ion-exchange chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurements and by reaction with anti-collagen type specific monoclonal antibodies.
Preservative Stabilizers
None present.
Reconstitution
Use 0.1 M acetic acid, pH 3.0. The dissolved collagen retains immunogenic properties. Thermal denaturation (5 min at 100 degree C) converts dissolved collagen to gelatin.
Buffer Solution
Essentially salt free
Preparation and Storage
Prior to reconstitution store at +4 degree C.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
Other Notes
Small volumes of COL5A2 purified protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for COL5A2 purified protein
ELISA (EIA)
NCBI/Uniprot data below describe general gene information for COL5A2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
Human Collagen Type I<1%
Human Collagen Type III<1%
Human Collagen Type IV<5%
Human Collagen Type VI<1%
[Other Products]
NCBI GenBank Nucleotide #
[Other Products]
UniProt Primary Accession #
[Other Products]
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
144,910 Da[Similar Products]
NCBI Official Full Name
collagen alpha-2(V) chain preproprotein
NCBI Official Synonym Full Names
collagen, type V, alpha 2
NCBI Official Symbol
COL5A2  [Similar Products]
NCBI Protein Information
collagen alpha-2(V) chain; collagen alpha-2(V) chain; AB collagen; type V preprocollagen alpha 2 chain; collagen, fetal membrane, A polypeptide
UniProt Protein Name
Collagen alpha-2(V) chain
Protein Family
UniProt Gene Name
COL5A2  [Similar Products]
UniProt Entry Name
CO5A2_HUMAN
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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