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IAA elisa kit :: Mouse IAA ELISA Kit

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Catalog # MBS2506102
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IAA, ELISA Kit

Popular Item
Full Product Name

Mouse IAA (Insulin Autoantibodies) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2506102 COA
3D Structure
ModBase 3D Structure for Q9FLN8
Species Reactivity
Specificity
This kit recognizes natural and recombinantMouse IAA. No significant cross-reactivity or interference between Mouse IAA and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IAA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIAApurchase
MBS2506102 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IAA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IAA. The ELISA analytical biochemical technique of the MBS2506102 kit is based on IAA antibody-IAA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IAA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IAA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IAA elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse IAA concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to IAA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for IAA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IAA, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IAA.You can calculate the concentration of IAA in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of IAA elisa kit
IAA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2506102. Click to request current manual
NCBI/Uniprot data below describe general gene information for IAA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,070 Da
NCBI Official Full Name
IAA carboxylmethyltransferase 1
NCBI Official Symbol
IAMT1  [Similar Products]
NCBI Official Synonym Symbols
IAA carboxylmethyltransferase 1; IAMT1; MCO15.20; MCO15_20
  [Similar Products]
NCBI Protein Information
IAA carboxylmethyltransferase 1
UniProt Protein Name
Indole-3-acetate O-methyltransferase 1
UniProt Synonym Protein Names
IAA carboxylmethyltransferase 1; S-adenosyl-L-methionine:(indol-3-yl) acetate carboxylmethyltransferase 1
Protein Family
UniProt Gene Name
IAMT1  [Similar Products]
UniProt Entry Name
IAMT1_ARATH
UniProt Comments for IAA
Catalyzes the methylation of the free carboxyl end of the plant hormone indole-3-acetic acid (IAA). Converts IAA to IAA methyl ester (MeIAA). Regulates IAA activities by IAA methylation. Methylation of IAA plays an important role in regulating plant development and auxin homeostasis. Required for correct leaf pattern formation. MeIAA seems to be an inactive form of IAA.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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