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ICAM1 elisa kit :: Rat ICAM-1 ELISA Kit

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Catalog # MBS175919
Unit / Price
  96-Strip-Wells  /  $455 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ICAM-1 (ICAM1), ELISA Kit

Popular Item
Full Product Name

Rat ICAM-1 PicoKine ELISA Kit

Product Synonym Names
Intercellular adhesion molecule 1; intercellular adhesion molecule 1; BB2; CD 54; CD54; CD54 antigen; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM 1; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MGC6195; MyD10; P3.58; Surface antigen of activated B cells
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 611162
3D Structure
ModBase 3D Structure for P05362
Species Reactivity
Specificity
Natural and recombinant rat ICAM-1
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 2pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ICAM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forICAM1purchase
MBS175919 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ICAM-1 (ICAM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ICAM1. The ELISA analytical biochemical technique of the MBS175919 kit is based on ICAM1 antibody-ICAM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ICAM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ICAM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ICAM1 elisa kit
Principle of the assay: rat ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-1 has been precoated onto 96-well plates. Standards(NSO,Q28 - T493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat ICAM-1 amount of sample captured in plate.
Background: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 Thenormal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cellsand its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of themajor histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 Thestandard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds.

Typical Testing Data/Standard Curve (for reference only) of ICAM1 elisa kit
Rat ICAM-1 PicoKine ELISA Kit standard curve
ICAM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175919. Click to request current manual
NCBI/Uniprot data below describe general gene information for ICAM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,825 Da
NCBI Official Full Name
Intercellular adhesion molecule 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
ICAM1  [Similar Products]
NCBI Official Synonym Symbols
BB2; CD54; P3.58
  [Similar Products]
NCBI Protein Information
intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Synonym Protein Names
Major group rhinovirus receptor; CD_antigen: CD54
UniProt Gene Name
ICAM1  [Similar Products]
UniProt Synonym Gene Names
ICAM-1  [Similar Products]
UniProt Entry Name
ICAM1_HUMAN
NCBI Summary for ICAM1
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
UniProt Comments for ICAM1
ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane

Molecular Function: integrin binding; viral receptor activity; protein binding; transmembrane receptor activity; receptor activity

Biological Process: extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; regulation of cell adhesion; response to drug; virion attachment, binding of host cell surface receptor; negative regulation of calcium ion transport; regulation of immune response; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; positive regulation of actin filament polymerization; response to copper ion; positive regulation of vasoconstriction; adhesion to host; cell adhesion mediated by integrin; response to ionizing radiation; leukocyte migration

Disease: Malaria, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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