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ICAM4 elisa kit :: Human ICAM4 ELISA Kit

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Catalog # MBS2503007
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ICAM4, ELISA Kit

Popular Item
Full Product Name

Human ICAM4 (Intercellular Adhesion Molecule 4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
111250
3D Structure
ModBase 3D Structure for Q14773
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ICAM4. No significant cross-reactivity or interference between Human ICAM4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ICAM4 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
3.125-200ng/mL
Sensitivity
Min: 1.875ng/mL; Max: 200ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ICAM4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forICAM4purchase
MBS2503007 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ICAM4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ICAM4. The ELISA analytical biochemical technique of the MBS2503007 kit is based on ICAM4 antibody-ICAM4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ICAM4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ICAM4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ICAM4 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ICAM4 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ICAM4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ICAM4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ICAM4. You can calculate the concentration of Human ICAM4 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ICAM4 elisa kit
ICAM4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2503007. Click to request current manual
NCBI/Uniprot data below describe general gene information for ICAM4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,649 Da
NCBI Official Full Name
intercellular adhesion molecule 4 isoform 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
NCBI Official Symbol
ICAM4  [Similar Products]
NCBI Official Synonym Symbols
LW; CD242
  [Similar Products]
NCBI Protein Information
intercellular adhesion molecule 4; CD242 antigen; LW blood group protein; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; intercellular adhesion molecule 4 (LW blood group); intercellular adhesion molecule 4 isoform 3 anitgen LWa
UniProt Protein Name
Intercellular adhesion molecule 4
UniProt Synonym Protein Names
Landsteiner-Wiener blood group glycoprotein; LW blood group protein; CD_antigen: CD242
UniProt Gene Name
ICAM4  [Similar Products]
UniProt Synonym Gene Names
LW; ICAM-4; LW blood group protein  [Similar Products]
UniProt Entry Name
ICAM4_HUMAN
NCBI Summary for ICAM4
This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. This ICAM protein contains 2 Ig-like C2-type domains and binds to the leukocyte adhesion LFA-1 protein. The molecular basis of the LW(A)/LW(B) blood group antigens is a single aa variation at position 100; Gln-100=LW(A) and Arg-100=LW(B). Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for ICAM4
ICAM4: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. Belongs to the immunoglobulin superfamily. ICAM family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2-cen

Disease: Blood Group System, Landsteiner-wiener
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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