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IFGF-23 elisa kit :: Mouse Intact Fibroblast Growth Factor 23 ELISA Kit

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Catalog # MBS748243
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Intact Fibroblast Growth Factor 23 (IFGF-23), ELISA Kit

Popular Item
Also Known As

Mouse Intact Fibroblast Growth Factor 23 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605380
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of iFGF-23. No significant cross-reactivity or interference between iFGF-23 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iFGF-23 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This iFGF-23 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse iFGF-23. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IFGF-23 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIFGF-23purchase
MBS748243 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Intact Fibroblast Growth Factor 23 (IFGF-23) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFGF-23. The ELISA analytical biochemical technique of the MBS748243 kit is based on IFGF-23 antibody-IFGF-23 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFGF-23 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFGF-23. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IFGF-23 elisa kit
Principle of the assay: iFGF-23 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-iFGF-23 antibody and an iFGF-23-HRP conjugate. The assay sample and buffer are incubated together with iFGF-23-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iFGF-23 concentration since iFGF-23 from samples and iFGF-23-HRP conjugate compete for the anti-iFGF-23 antibody binding site. Since the number of sites is limited, as more sites are occupied by iFGF-23 from the sample, fewer sites are left to bind iFGF-23-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iFGF-23 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IFGF-23 elisa kit

Typical Testing Data/Standard Curve (for reference only) of IFGF-23 elisa kit
IFGF-23 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS748243. Click to request current manual
NCBI/Uniprot data below describe general gene information for IFGF-23. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,954 Da
NCBI Official Full Name
FGF23
NCBI Official Synonym Full Names
fibroblast growth factor 23
NCBI Official Symbol
FGF23  [Similar Products]
NCBI Official Synonym Symbols
ADHR; FGFN; HYPF; HPDR2; PHPTC
  [Similar Products]
NCBI Protein Information
fibroblast growth factor 23; phosphatonin; tumor-derived hypophosphatemia inducing factor
UniProt Protein Name
Fibroblast growth factor 23
UniProt Synonym Protein Names
Phosphatonin; Tumor-derived hypophosphatemia-inducing factorCleaved into the following 2 chains:Fibroblast growth factor 23 N-terminal peptide; Fibroblast growth factor 23 C-terminal peptide
UniProt Gene Name
FGF23  [Similar Products]
UniProt Synonym Gene Names
HYPF; UNQ3027/PRO9828; FGF-23  [Similar Products]
UniProt Entry Name
FGF23_HUMAN
NCBI Summary for IFGF-23
This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013]
UniProt Comments for IFGF-23
FGF23: Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR). ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses. Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; type 1 fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; negative regulation of hormone secretion; positive regulation of transcription, DNA-dependent; phosphate metabolic process; negative regulation of bone mineralization; cellular phosphate ion homeostasis; insulin receptor signaling pathway; innate immune response; negative regulation of osteoblast differentiation; phosphate ion homeostasis; cell differentiation; vitamin D catabolic process

Disease: Hypophosphatemic Rickets, Autosomal Dominant
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with IFGF-23 elisa kit
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