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IFITM3 elisa kit :: Human Interferon-induced transmembrane protein 3 ELISA Kit

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Catalog # MBS763456
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Interferon-induced transmembrane protein 3 (IFITM3), ELISA Kit

Popular Item
Also Known As

Human Interferon-induced transmembrane protein 3 ELISA Kit

Product Synonym Names
IFITM3/Fragilis/IP15/mil-1/1-8U/DSPA2b/interferon induced transmembrane protein 3/interferon-induced transmembrane protein 3/Interferon-inducible protein 1-8U
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS763456 COA
Sequence Length
133
OMIM
605579
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of IFITM3. No significant cross-reactivity or interference between IFITM3 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.2-2000pg/ml
Sensitivity
<18.75pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFITM3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFITM3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IFITM3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIFITM3purchase
MBS763456 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon-induced transmembrane protein 3 (IFITM3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFITM3. The ELISA analytical biochemical technique of the MBS763456 kit is based on IFITM3 antibody-IFITM3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFITM3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFITM3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IFITM3 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IFITM3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IFITM3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IFITM3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IFITM3 can be calculated.

Typical Standard Curve/Testing Data of IFITM3 elisa kit
IFITM3 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS763456. Click to request current manual
NCBI/Uniprot data below describe general gene information for IFITM3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,632 Da
NCBI Official Full Name
interferon-induced transmembrane protein 3
NCBI Official Synonym Full Names
interferon induced transmembrane protein 3
NCBI Official Symbol
IFITM3  [Similar Products]
NCBI Official Synonym Symbols
1-8U; IP15; DSPA2b
  [Similar Products]
NCBI Protein Information
interferon-induced transmembrane protein 3
UniProt Protein Name
Interferon-induced transmembrane protein 3
UniProt Synonym Protein Names
Dispanin subfamily A member 2b; DSPA2b; Interferon-inducible protein 1-8U
UniProt Gene Name
IFITM3  [Similar Products]
UniProt Synonym Gene Names
DSPA2b  [Similar Products]
UniProt Entry Name
IFM3_HUMAN
NCBI Summary for IFITM3
The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. [provided by RefSeq, May 2012]
UniProt Comments for IFITM3
IFITM3: IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. Interacts with SPP1; the interaction reduces OPN expression. By IFN-alpha and IFNG/IFN-gamma. Belongs to the CD225/Dispanin family.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: integral to membrane; late endosome membrane; lysosomal membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; immune response; negative regulation of viral genome replication; negative regulation of viral transcription; negative regulation of virion penetration into host cell; response to virus

Disease: Influenza, Severe, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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