• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IFN-alpha-Ab elisa kit :: Mouse IFN-alpha-Ab ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2511130
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IFN-alpha-Ab, ELISA Kit

Popular Item
Full Product Name

Mouse IFN-alpha-Ab (Interferon-alpha Antibody) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
147578
3D Structure
ModBase 3D Structure for P01562
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse IFN-alpha-Ab. No significant cross-reactivity or interference between Mouse IFN-alpha-Ab and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse IFN-alpha-Ab and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IFN-alpha-Ab elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIFN-alpha-Abpurchase
MBS2511130 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IFN-alpha-Ab, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFN-alpha-Ab. The ELISA analytical biochemical technique of the MBS2511130 kit is based on IFN-alpha-Ab antibody-IFN-alpha-Ab antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFN-alpha-Ab antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFN-alpha-Ab. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IFN-alpha-Ab elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFN-alpha-Ab in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-alpha-Ab. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-alpha-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-alpha-Ab, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IFN-alpha-Ab. You can calculate the concentration of Mouse IFN-alpha-Ab in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of IFN-alpha-Ab elisa kit
IFN-alpha-Ab elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2511130. Click to request current manual
NCBI/Uniprot data below describe general gene information for IFN-alpha-Ab. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,725 Da
NCBI Official Full Name
interferon alpha-1/13
NCBI Official Synonym Full Names
interferon, alpha 1
NCBI Official Symbol
IFNA1  [Similar Products]
NCBI Official Synonym Symbols
IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
  [Similar Products]
NCBI Protein Information
interferon alpha-1/13; IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; interferon alpha-D; interferon-alpha1; leIF D
UniProt Protein Name
Interferon alpha-1/13
UniProt Synonym Protein Names
Interferon alpha-D; LeIF D
UniProt Gene Name
IFNA1  [Similar Products]
UniProt Synonym Gene Names
IFN-alpha-1/13; LeIF D  [Similar Products]
UniProt Entry Name
IFNA1_HUMAN
NCBI Summary for IFN-alpha-Ab
The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]
UniProt Comments for IFN-alpha-Ab
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; natural killer cell activation during immune response; regulation of MHC class I biosynthetic process; innate immune response; blood coagulation; defense response to virus; humoral immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; T cell activation during immune response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000