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IFN-gamma elisa kit :: Rat IFN-gamma ELISA Kit

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Catalog # MBS2500392 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  48-Strip-Wells  /  $310 +1 FREE 8GB USB
  96-Strip-Wells  /  $355 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
  10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IFN-gamma, ELISA Kit

Popular Item
Full Product Name

Rat IFN-gamma (Interferon Gamma) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2500392 COA
OMIM
147570
3D Structure
ModBase 3D Structure for P01579
Species Reactivity
Specificity
This kit recognizes natural and some recombinant Rat IFN-?. No significant crossreactivity or interference between Rat IFN-? and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rat IFN-? were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rat IFN-? were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IFN-gamma elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIFN-gammapurchase
MBS2500392 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IFN-gamma, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFN-gamma. The ELISA analytical biochemical technique of the MBS2500392 kit is based on IFN-gamma antibody-IFN-gamma antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFN-gamma antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFN-gamma. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IFN-gamma elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat IFN-? concentrations in serum, plasma and other biological fluids.

Typical Testing Data/Standard Curve (for reference only) of IFN-gamma elisa kit
IFN-gamma elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2500392. Click to request current manual
NCBI/Uniprot data below describe general gene information for IFN-gamma. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,348 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
NCBI Official Synonym Symbols
IFG; IFI
  [Similar Products]
NCBI Protein Information
interferon gamma; IFN-gamma; immune interferon
UniProt Protein Name
Interferon gamma
UniProt Synonym Protein Names
Immune interferon
UniProt Gene Name
UniProt Synonym Gene Names
IFN-gamma  [Similar Products]
UniProt Entry Name
IFNG_HUMAN
NCBI Summary for IFN-gamma
This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]
UniProt Comments for IFN-gamma
IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Secreted, signal peptide; Secreted; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-23 production; positive regulation of interleukin-12 production; apoptosis; positive regulation of osteoclast differentiation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; positive regulation of interleukin-1 beta secretion; sensory perception of mechanical stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion

Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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