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IFNG elisa kit :: Mouse interferon, gamma ELISA Kit

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Catalog # MBS704703
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

interferon, gamma (IFNG), ELISA Kit

Popular Item
Also Known As

Mouse Interferon gamma, IFN-gamma ELISA Kit

Product Synonym Names
Mouse Interferon gamma; IFN-gamma ELISA Kit; IFG; IFI; ; interferon; gamma
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704703 Testing Data
Sequence Length
155
3D Structure
ModBase 3D Structure for P01580
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse IFNG. No significant cross-reactivity or interference between Mouse IFNG and analogues was observed.
Samples
Serum, plasma, tissue homogenates and cell culture supernates
Assay Type
Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Immunity
Protein Biological Process 3
Antiviral defense
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IFNG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIFNGpurchase
MBS704703 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interferon, gamma (IFNG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFNG. The ELISA analytical biochemical technique of the MBS704703 kit is based on IFNG antibody-IFNG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFNG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFNG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IFNG elisa kit
For Sample For the quantitative determination of mouse interferon ? (IFN mouse interferon concentrations in serum, plasma, tissue homogenates and cell culture supernates.

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFN-? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-? bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of IFNG elisa kit
IFNG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704703. Click to request current manual
NCBI/Uniprot data below describe general gene information for IFNG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,907 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon gamma
NCBI Official Symbol
NCBI Official Synonym Symbols
Ifg; IFN-g
  [Similar Products]
NCBI Protein Information
interferon gamma; IFN-gamma; gamma interferon
UniProt Protein Name
Interferon gamma
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IFN-gamma  [Similar Products]
UniProt Entry Name
IFNG_MOUSE
NCBI Summary for IFNG
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
UniProt Comments for IFNG
Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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