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IGF-I active protein

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Catalog # MBS506125
Unit / Price
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  0.02 mg  /  $140 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  1 mg  /  $315 +1 FREE 8GB USB
  5 mg  /  $1,215 +1 FREE 8GB USB
Product Name

IGF-I, Active Protein

Popular Item
Full Product Name

Recombinant Human IGF-I

Product Synonym Names
Human IGF-I (IGF-1); Insulin-like Growth Factor I; Somatamedin C; mechano growth factor; IGF-IA
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
OMIM
X00173 mRNA
3D Structure
ModBase 3D Structure for P05019
Host
E Coli
Purity/Purification
Purity is typically 97% as determined by reducing and non-reducing SDS-PAGE
Form/Format
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing
0.1% Trifluoroacetic Acid (TFA)
Species
Human
Bioactivity Assay
FDC-P1 cell proliferation
Bioactivity Acceptance Criteria ED50 (ng/mL)
10 ng/mL
Bioactivity Acceptance Criteria (Units/mg)
1.0 x 10^5 units/mg
Reconstitution
Sterile water at 0.1 mg/mL
Acceptance Criteria LAL (EUs/ug)
<=1.00
Monomer/Dimer
Monomer
Endotoxin Level (kinetic LAL analysis)
Typically less than 0.05 EUs/mug protein (50% confidence range)
Biological Activity
The activity is determined by the dose-dependent proliferation of mouse FDC-P1 cells is typically less than 1.0 ng/mL. This corresponds to an expected specific activity of 1 x 106 units/mg.
Media Components
Luria Broth
Preparation and Storage
12 months from date of receipt when stored at -20 degree C to-80 degree C as supplied.
1 month when stored at 4 degree C after reconstituting as directed.
3 months when stored at -20 degree C to -80 degree C after reconstituting as directed.
Active Protein Note
Refer to the datasheet for activity details. For other activity applications, researchers should evaluate this protein for suitability in their model system.
Other Notes
Small volumes of IGF-I active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IGF-I active protein
Insulin-like growth factor I (IGF-I) is a growth factor that is produced by the liver. IGF-1 production is stimulated by growth hormone (GH). IGF-I binds the insulin-like growth factor 1 receptor (IGF1R) and the insulin receptor to stimulate systemic body growth. IGF-I is one of the most potent activators of the AKT signaling pathway, which stimulates cell proliferation and inhibits programmed cell death. Mature human IGF-I is 100% homologous to bovine and porcine IGF-1 proteins.

IGF-I active protein Gel Data image
Biological Activity of IGF-I active protein
IGF-I active protein Biological Activity image
NCBI/Uniprot data below describe general gene information for IGF-I. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Non-glycosylated protein, containing 70 amino acids, with a molecular weight of 7.7 kDa.
NCBI Official Full Name
insulin-like growth factor I isoform 4 preproprotein
NCBI Official Synonym Full Names
insulin like growth factor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
MGF; IGFI; IGF-I
  [Similar Products]
NCBI Protein Information
insulin-like growth factor I
UniProt Protein Name
Insulin-like growth factor I
UniProt Synonym Protein Names
Mechano growth factor; MGF; Somatomedin-C
UniProt Gene Name
UniProt Synonym Gene Names
IBP1; IGF-I; MGF  [Similar Products]
NCBI Summary for IGF-I
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
UniProt Comments for IGF-I
IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: extracellular region; extracellular space; insulin-like growth factor binding protein complex; plasma membrane

Molecular Function: hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; integrin binding; protein binding

Biological Process: activation of MAPK activity; activation of protein kinase B; cell activation; cell motility; cell proliferation; cellular protein metabolic process; DNA replication; glycolate metabolic process; insulin-like growth factor receptor signaling pathway; muscle development; muscle hypertrophy; myoblast differentiation; myoblast proliferation; myotube cell development; negative regulation of apoptosis; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; platelet degranulation; positive regulation of activated T cell proliferation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of glycolysis; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein import into nucleus, translocation; positive regulation of protein secretion; positive regulation of Ras protein signal transduction; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein; protein kinase B signaling cascade; protein stabilization; proteoglycan biosynthetic process; Ras protein signal transduction; regulation of gene expression; regulation of multicellular organism growth; response to heat; satellite cell compartment self-renewal involved in skeletal muscle regeneration; signal transduction; skeletal development

Disease: Insulin-like Growth Factor I Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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