• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IGF1 recombinant protein :: IGF-I Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS400008
Unit / Price
  0.01 mg  /  $155 +1 FREE 8GB USB
  0.05 mg  /  $240 +1 FREE 8GB USB
  0.1 mg  /  $330 +1 FREE 8GB USB
  0.5 mg  /  $920 +1 FREE 8GB USB
  1 mg  /  $1,585 +1 FREE 8GB USB
  5 mg  /  $6,205 +7 FREE 8GB USB
IGF1 recombinant protein
Product Name

IGF-I (IGF1), Recombinant Protein

Popular Item
Also Known As

Fish (denis) IGF-I

Product Synonym Names
Fish gilthead seabream (Sparus aurata) IGF-I
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
137
Sequence
The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ser-Pr o-Glu-Thr- Leu-Cys-Gl y-Ala-Glu.
OMIM
147440
3D Structure
ModBase 3D Structure for P05019
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
Form/Format
White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized gsIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.60 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.
Dimers and Aggregates
The purified gsIGF-I consists of > 97% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of Gilthead Seabream IGF-I
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Biological Activity
Binding assays of the 125I-gsIGF-I to gilthead seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude gilthead seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF-I was about fourfold lower than that of gsIGF-I. Recombinant gsIGF-I exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF-1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF-I receptor in these cells. In contrast, the activities of gsIGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant gsIGF-I is biologically active.
Preparation and Storage
Lyophilized gsIGF-I although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 gsIGF-I mg/ml and up to 2 2 mg/ml and filter sterilization gsIGF-I can be stored at +4 degree C
Other Notes
Small volumes of IGF1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IGF1 recombinant protein
Gilthead seabream (Sparus aurata) insulin-like growth factor-I (gsIGF-I) cDNA coding the mature protein was expressed in Escherichia coli. The expressed protein contained within the inclusion-body pellet was solubilized and purified to over 98% purity, as a monomeric methionyl-gsIGF-I. Amino acid composition and N-terminal sequence confirmed the identity to be the predicted protein. For reference see Fine et al., J Endocrinol. 1997 53:139-150.
NCBI/Uniprot data below describe general gene information for IGF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,762 Da[Similar Products]
NCBI Official Full Name
insulin-like growth factor I
NCBI Official Synonym Full Names
insulin-like growth factor 1 (somatomedin C)
NCBI Official Symbol
NCBI Official Synonym Symbols
IGFI; IGF-I; IGF1A
  [Similar Products]
NCBI Protein Information
insulin-like growth factor I; IGF-IA; IGF-IB; MGF; insulin-like growth factor IA; insulin-like growth factor IB; mechano growth factor; somatomedin-C
UniProt Protein Name
Insulin-like growth factor I
UniProt Synonym Protein Names
Mechano growth factor; MGF; Somatomedin-C
UniProt Gene Name
UniProt Synonym Gene Names
IBP1; IGF-I; MGF  [Similar Products]
UniProt Entry Name
IGF1_HUMAN
NCBI Summary for IGF1
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
UniProt Comments for IGF1
IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; plasma membrane; extracellular region

Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding

Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; glycolate metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of mitosis; positive regulation of protein import into nucleus, translocation; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; branching morphogenesis of a tube; regulation of gene expression; response to heat; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; myoblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of glycogen biosynthetic process; signal transduction; positive regulation of smooth muscle cell migration; positive regulation of activated T cell proliferation; negative regulation of cell proliferation; glial cell differentiation; platelet degranulation; mammary gland development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; myotube cell development; regulation of multicellular organism growth; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cell proliferation; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; Ras protein signal transduction; positive regulation of glycolysis; blood vessel remodeling; positive regulation of Ras protein signal transduction; blood coagulation; cell motility; positive regulation of DNA replication

Disease: Insulin-like Growth Factor I Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000