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IGF1R elisa kit :: Sheep Insulin-like growth factor 1 receptor (IGF1R) ELISA Kit

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Catalog # MBS7236768
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Insulin-like growth factor 1 receptor (IGF1R), ELISA Kit

Popular Item
Also Known As

Sheep Insulin-like growth factor 1 receptor (IGF1R) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 270450
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of IGF-1R. No significant cross-reactivity or interference between IGF-1R and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGF-1R and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IGF1R elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIGF1Rpurchase
MBS7236768 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Insulin-like growth factor 1 receptor (IGF1R) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGF1R. The ELISA analytical biochemical technique of the MBS7236768 kit is based on IGF1R antibody-IGF1R antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGF1R antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGF1R. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IGF1R elisa kit
Intended Uses: This IGF-1R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IGF-1R. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||IGF-1R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF-1R antibody and an IGF-1R-HRP conjugate. The assay sample and buffer are incubated together with IGF-1R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF-1R concentration since IGF-1R from samples and IGF-1R-HRP conjugate compete for the anti-IGF-1R antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF-1R from the sample, fewer sites are left to bind IGF-1R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF-1R concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IGF1R elisa kit

Typical Testing Data/Standard Curve of IGF1R elisa kit
IGF1R elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS7236768. Click to request current manual
NCBI/Uniprot data below describe general gene information for IGF1R. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
154,793 Da
NCBI Official Full Name
Insulin-like growth factor 1 receptor
NCBI Official Synonym Full Names
insulin-like growth factor 1 receptor
NCBI Official Symbol
IGF1R  [Similar Products]
NCBI Official Synonym Symbols
IGFR; CD221; IGFIR; JTK13
  [Similar Products]
NCBI Protein Information
insulin-like growth factor 1 receptor; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor
UniProt Protein Name
Insulin-like growth factor 1 receptor
UniProt Synonym Protein Names
Insulin-like growth factor I receptor; IGF-I receptor; CD_antigen: CD221Cleaved into the following 2 chains:Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain
UniProt Gene Name
IGF1R  [Similar Products]
UniProt Synonym Gene Names
IGF-I receptor  [Similar Products]
UniProt Entry Name
IGF1R_HUMAN
NCBI Summary for IGF1R
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for IGF1R
IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: neuron projection; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; caveola; receptor complex

Molecular Function: insulin binding; insulin-like growth factor binding; identical protein binding; protein binding; insulin-like growth factor I binding; insulin receptor substrate binding; protein-tyrosine kinase activity; phosphoinositide 3-kinase binding; G-protein alpha-subunit binding; ATP binding; insulin receptor binding; insulin-like growth factor receptor activity

Biological Process: epidermis development; phosphoinositide 3-kinase cascade; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; mammary gland development; positive regulation of MAPKKK cascade; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; insulin-like growth factor receptor signaling pathway; axonogenesis; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; positive regulation of cell migration; negative regulation of apoptosis

Disease: Insulin-like Growth Factor I, Resistance To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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