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IGF2R sirna

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Catalog # MBS8214144
Unit / Price
  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
IGF2R sirna
Product Name

IGF2R, siRNA

Popular Item
Full Product Name

IGF2R siRNA (Mouse)

Product Synonym Names
Cation-independent mannose-6-phosphate receptor; CI Man-6-P receptor; CI-MPR; M6PR; 300 kDa mannose 6-phosphate receptor; MPR 300; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; IGF-II receptor; M6P/IGF2 receptor; M6P/IGF2R; CD antigen CD222
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q07113
Host
Synthetic
Species Reactivity
Mouse
Specificity
IGF2R siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IGF2R gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of IGF2R sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IGF2R sirna
siRNA to inhibit IGF2R expression using RNA interference
Applications Tested/Suitable for IGF2R sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for IGF2R. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
273,815 Da
NCBI Official Full Name
cation-independent mannose-6-phosphate receptor
NCBI Official Synonym Full Names
insulin-like growth factor 2 receptor
NCBI Official Symbol
Igf2r  [Similar Products]
NCBI Official Synonym Symbols
CD222; CI-MPR; Mpr300; AI661837; M6P/IGF2R
  [Similar Products]
NCBI Protein Information
cation-independent mannose-6-phosphate receptor
UniProt Protein Name
Cation-independent mannose-6-phosphate receptor
UniProt Synonym Protein Names
300 kDa mannose 6-phosphate receptor; MPR 300; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; IGF-II receptor; M6P/IGF2 receptor; M6P/IGF2R; CD_antigen: CD222
UniProt Gene Name
Igf2r  [Similar Products]
UniProt Synonym Gene Names
CI Man-6-P receptor; CI-MPR; M6PR; MPR 300; IGF-II receptor; M6P/IGF2R  [Similar Products]
UniProt Entry Name
MPRI_MOUSE
UniProt Comments for IGF2R
IGF2R: a multifunctional type I transmembrane protein receptor that binds insulin-like growth factor 2 at the cell surface and mannose-6-phosphate-tagged proteins in the trans-Golgi network. Also known as the cation-independent mannose-6 phosphate (M6P) receptor. Clears IGF2 from the cell surface to attenuate signaling, and transports M6P-tagged lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. M6P-tagged lysosomal enzymes bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Belongs to the MRL1/IGF2R family.

Protein type: Vesicle; Receptor, misc.; Membrane protein, integral

Cellular Component: extracellular space; focal adhesion; cell surface; clathrin coat; lysosome; integral to membrane; trans-Golgi network; trans-Golgi network transport vesicle; membrane; endocytic vesicle; nuclear envelope lumen; perinuclear region of cytoplasm; late endosome; cytoplasm; plasma membrane; nucleus; endosome

Molecular Function: G-protein coupled receptor activity; insulin-like growth factor binding; identical protein binding; mannose binding; enzyme binding; retinoic acid binding; transporter activity; G-protein alpha-subunit binding; phosphoprotein binding; glycoprotein binding; insulin-like growth factor II binding

Biological Process: regulation of apoptosis; G-protein coupled receptor protein signaling pathway; response to retinoic acid; transport; positive regulation of apoptosis; liver development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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