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IGFBP-3 elisa kit :: Rat IGFBP-3 ELISA Kit

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Catalog # MBS2515458
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IGFBP-3, ELISA Kit

Popular Item
Also Known As

Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2515458 COA
MBS2515458 QC Report
Sequence Length
291
OMIM
146732
3D Structure
ModBase 3D Structure for P17936
Species Reactivity
Specificity
This kit recognizes natural and some recombinant Rat IGFBP-3. No significant crossreactivity or interference between Rat IGFBP-3 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
Min: 46.875pg/mL; Max: 5000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IGFBP-3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIGFBP-3purchase
MBS2515458 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IGFBP-3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP-3. The ELISA analytical biochemical technique of the MBS2515458 kit is based on IGFBP-3 antibody-IGFBP-3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP-3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP-3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IGFBP-3 elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat IGFBP-3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat IGFBP-3. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IGFBP-3. The concentration of Rat IGFBP-3 in samples can be calculated by comparing the OD of the samples with the standard curve.

Typical Testing Data/Standard Curve (for reference only) of IGFBP-3 elisa kit
IGFBP-3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2515458. Click to request current manual
NCBI/Uniprot data below describe general gene information for IGFBP-3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
32,223 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 3 isoform b
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 3
NCBI Official Symbol
IGFBP3  [Similar Products]
NCBI Official Synonym Symbols
IBP3; BP-53
  [Similar Products]
NCBI Protein Information
insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein
UniProt Protein Name
Insulin-like growth factor-binding protein 3
UniProt Gene Name
IGFBP3  [Similar Products]
UniProt Synonym Gene Names
IBP3; IBP-3; IGF-binding protein 3; IGFBP-3  [Similar Products]
UniProt Entry Name
IBP3_HUMAN
NCBI Summary for IGFBP-3
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for IGFBP-3
IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region; nucleus

Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; metal ion binding; fibronectin binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity

Biological Process: positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; regulation of insulin-like growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; negative regulation of smooth muscle cell proliferation; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; negative regulation of signal transduction; osteoblast differentiation; negative regulation of cell proliferation; cellular protein metabolic process; negative regulation of protein amino acid phosphorylation; positive regulation of MAPKKK cascade; regulation of cell growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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