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IGFBP-5 elisa kit :: Human IGFBP-5 ELISA Kit

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Catalog # MBS2503629
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IGFBP-5, ELISA Kit

Popular Item
Full Product Name

Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
146734
3D Structure
ModBase 3D Structure for P24593
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human IGFBP-5. No significant cross-reactivity or interference between Human IGFBP-5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human IGFBP-5 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IGFBP-5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IGFBP-5purchase
MBS2503629 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IGFBP-5, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP-5. The ELISA analytical biochemical technique of the MBS2503629 kit is based on IGFBP-5 antibody-IGFBP-5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP-5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP-5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IGFBP-5 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGFBP-5 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IGFBP-5. You can calculate the concentration of Human IGFBP-5 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of IGFBP-5 elisa kit
IGFBP-5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2503629. Click to request current manual
NCBI/Uniprot data below describe general gene information for IGFBP-5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,570 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 5
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 5
NCBI Official Symbol
IGFBP5  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5
UniProt Protein Name
Insulin-like growth factor-binding protein 5
UniProt Gene Name
IGFBP5  [Similar Products]
UniProt Synonym Gene Names
IBP5; IBP-5; IGF-binding protein 5; IGFBP-5  [Similar Products]
UniProt Entry Name
IBP5_HUMAN
UniProt Comments for IGFBP-5
IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region

Molecular Function: protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; mammary gland involution; hair follicle morphogenesis; negative regulation of smooth muscle cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; glucose metabolic process; negative regulation of smooth muscle cell migration; glucose homeostasis; signal transduction; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; negative regulation of translation; striated muscle cell differentiation; negative regulation of osteoblast differentiation; regulation of cell growth; skeletal muscle growth; negative regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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