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IGFBP1 elisa kit :: Human insulin-like growth factor binding protein 1 ELISA Kit

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Catalog # MBS704929 (SPECIAL PROMOTIONAL PRICING for a limited time)
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Typical Testing Data/Standard Curve (for reference only)
Product Name

insulin-like growth factor binding protein 1 (IGFBP1), ELISA Kit

Popular Item
Full Product Name

Human insulin-like growth factors binding protein 1, IGFBP-1 ELISA Kit

Product Synonym Names
Human insulin-like growth factors binding protein 1; IGFBP-1 ELISA Kit; AFBP; IBP1; IGF-BP25; PP12; hIGFBP-1; IGF-binding protein 1; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; gr; insulin-like growth factor binding protein 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704929 Testing Data
OMIM
146730
3D Structure
ModBase 3D Structure for P08833
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human IGFBP1. No significant cross-reactivity or interference between Human IGFBP1 and analogues was observed.
Samples
Serum, plasma and tissue homogenates
Assay Type
Sandwich
Detection Range
3.12 ng/ml-200 ng/ml
Sensitivity
0.78 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IGFBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IGFBP1purchase
MBS704929 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the insulin-like growth factor binding protein 1 (IGFBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP1. The ELISA analytical biochemical technique of the MBS704929 kit is based on IGFBP1 antibody-IGFBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IGFBP1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGFBP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGFBP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGFBP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of IGFBP1 elisa kit
IGFBP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704929. Click to request current manual
NCBI/Uniprot data below describe general gene information for IGFBP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,904 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 1
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 1
NCBI Official Symbol
IGFBP1  [Similar Products]
NCBI Official Synonym Symbols
AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
  [Similar Products]
NCBI Protein Information
insulin-like growth factor-binding protein 1; IBP-1; IGFBP-1; binding protein-25; binding protein-26; binding protein-28; placental protein 12; IGF-binding protein 1; amniotic fluid binding protein; growth hormone independent-binding protein; alpha-pregnancy-associated endometrial globulin
UniProt Protein Name
Insulin-like growth factor-binding protein 1
UniProt Synonym Protein Names
Placental protein 12; PP12
UniProt Gene Name
IGFBP1  [Similar Products]
UniProt Synonym Gene Names
IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12  [Similar Products]
UniProt Entry Name
IBP1_HUMAN
NCBI Summary for IGFBP1
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
UniProt Comments for IGFBP1
Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.1 PublicationManual assertion based on experiment in:Ref.20
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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