Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List IGFBP1 elisa kit :: Mouse IGFBP-1 ELISA Kit Catalog # MBS175824 Unit / Price 96-Strip-Wells / $440 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name IGFBP-1 (IGFBP1), ELISA Kit Popular Item Full Product Name Mouse IGFBP-1 PicoKine ELISA Kit Product Synonym Names Insulin-like growth factor-binding protein 1; insulin-like growth factor binding protein 1; AFBP; hIGFBP-1; IBP1; IGF-binding protein 1; IGF-BP25; Insulin-like growth factor-binding protein 1; Placental protein 12; PP12 Product Gene Name IGFBP1 elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Request for Current Manual Insert Request Current Manual 3D Structure ModBase 3D Structure for P47876 Species Reactivity Mouse Specificity Natural and recombinant mouse IGFBP-1 Samples Cell culture supernates, serum and plasma(heparin, EDTA). Detection Range 31.2pg/ml-2000pg/ml Sensitivity < 3pg/ml Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Preparation and Storage Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) ISO Certification Manufactured in an ISO 9001:2008 Certified Laboratory. Product Note Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual. Other Notes Small volumes of IGFBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms forIGFBP1purchase MBS175824 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IGFBP-1 (IGFBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP1. The ELISA analytical biochemical technique of the MBS175824 kit is based on IGFBP1 antibody-IGFBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for IGFBP1 elisa kit Principle of the Assay: The mouse IGFBP-1 ELISA Kit was based on standard sanuwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A26-N272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then fOllowed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP-1 amount of sample captured in plate.Background: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and 11.3 The gene is organized in four exons and spans 5.9 kb. 4 The IBP-1 gene is a single copy gene, located on chromosome 7.5 The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KOa. Typical Testing Data/Standard Curve (for reference only) of IGFBP1 elisa kit Mouse IGFBP-1 PicoKine ELISA Kit standard curve TOP Sample Manual Insert of MBS175824. Click to request current manual NCBI/Uniprot data below describe general gene information for IGFBP1. It may not necessarily be applicable to this product. NCBI GI # 408360142 NCBI GeneID 16006 NCBI Accession # P47876.2 [Other Products] UniProt Primary Accession # P47876 [Other Products] UniProt Secondary Accession # Q5SVY8; Q61732[Other Products] UniProt Related Accession # P47876[Other Products] Molecular Weight 29,570 Da TOP NCBI Official Full Name Insulin-like growth factor-binding protein 1 NCBI Official Synonym Full Names insulin-like growth factor binding protein 1 NCBI Official Symbol Igfbp1 [Similar Products] NCBI Protein Information insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1 PRECURSOR (IGFBP-1) (IBP-1) (IGF-BINDING PROTEIN 1) UniProt Protein Name Insulin-like growth factor-binding protein 1 Protein Family Insulin-like growth factor-binding protein UniProt Gene Name Igfbp1 [Similar Products] UniProt Synonym Gene Names Igfbp-1; IBP-1; IGF-binding protein 1; IGFBP-1 [Similar Products] UniProt Entry Name IBP1_MOUSE TOP TOP UniProt Comments for IGFBP1 IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.Protein type: Secreted, signal peptide; SecretedCellular Component: extracellular space; extracellular regionMolecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; growth factor binding; insulin-like growth factor II bindingBiological Process: regulation of insulin-like growth factor receptor signaling pathway; regulation of cell growth Research Articles on IGFBP1 1. serum IGFBPI protein could provide an easily measured biomarker for early detection of alcohol-induced liver injury in humans TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated withIGFBP1 elisa kit Reference Product PubMed Publications Igf1 elisa kit >72 publications with IGFBP1 and Igf1 Igf2 elisa kit >33 publications with IGFBP1 and Igf2 Egf elisa kit >23 publications with IGFBP1 and Egf Il6 elisa kit >10 publications with IGFBP1 and Il6 Foxa2 elisa kit >8 publications with IGFBP1 and Foxa2 Stat3 elisa kit >7 publications with IGFBP1 and Stat3 Foxo4 elisa kit >3 publications with IGFBP1 and Foxo4 Atf4 elisa kit >3 publications with IGFBP1 and Atf4 Ins2 elisa kit >1 publications with IGFBP1 and Ins2 Pathways associated with IGFBP1 elisa kit Products by Pathway Pathway Diagram ATF4 Activates Genes Pathway antibodies ATF4 Activates Genes Pathway Diagram Metabolism Of Proteins Pathway antibodies Metabolism Of Proteins Pathway Diagram Myometrial Relaxation And Contraction Pathways antibodies Myometrial Relaxation And Contraction Pathways Diagram PERK Regulates Gene Expression Pathway antibodies PERK Regulates Gene Expression Pathway Diagram Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram Unfolded Protein Response (UPR) Pathway antibodies Unfolded Protein Response (UPR) Pathway Diagram Diseases associated with IGFBP1 elisa kit Disease Name Pubmed Publications Liver Diseases Antibodies >129 publications with IGFBP1 and Liver Diseases Cardiovascular Diseases Antibodies >122 publications with IGFBP1 and Cardiovascular Diseases Disease Models, Animal Antibodies >90 publications with IGFBP1 and Disease Models, Animal Kidney Diseases Antibodies >83 publications with IGFBP1 and Kidney Diseases Liver Neoplasms Antibodies >81 publications with IGFBP1 and Liver Neoplasms Adenocarcinoma Antibodies >80 publications with IGFBP1 and Adenocarcinoma Brain Diseases Antibodies >75 publications with IGFBP1 and Brain Diseases Necrosis Antibodies >65 publications with IGFBP1 and Necrosis Hyperglycemia Antibodies >56 publications with IGFBP1 and Hyperglycemia Carcinoma, Hepatocellular Antibodies >54 publications with IGFBP1 and Carcinoma, Hepatocellular Organs/Tissues associated with IGFBP1 elisa kit Organ/Tissue Name Pubmed Publications Liver Antibodies >449 publications with IGFBP1 and Liver Kidney Antibodies >158 publications with IGFBP1 and Kidney Muscle Antibodies >150 publications with IGFBP1 and Muscle Embryonic Tissue Antibodies >130 publications with IGFBP1 and Embryonic Tissue Lung Antibodies >46 publications with IGFBP1 and Lung Extraembryonic Tissue Antibodies >9 publications with IGFBP1 and Extraembryonic Tissue Uncharacterized Tissue Antibodies >1 publications with IGFBP1 and Uncharacterized Tissue TOP © 2006-18 MyBioSource.com. 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