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IGFBP1 elisa kit :: Mouse IGFBP-1 ELISA Kit

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Catalog # MBS175824
Unit / Price
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  96-Strip-Wells  /  $440 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IGFBP-1 (IGFBP1), ELISA Kit

Popular Item
Full Product Name

Mouse IGFBP-1 PicoKine ELISA Kit

Product Synonym Names
Insulin-like growth factor-binding protein 1; insulin-like growth factor binding protein 1; AFBP; hIGFBP-1; IBP1; IGF-binding protein 1; IGF-BP25; Insulin-like growth factor-binding protein 1; Placental protein 12; PP12
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for P47876
Species Reactivity
Specificity
Natural and recombinant mouse IGFBP-1
Samples
Cell culture supernates, serum and plasma(heparin, EDTA).
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 3pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IGFBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IGFBP1purchase
MBS175824 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IGFBP-1 (IGFBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP1. The ELISA analytical biochemical technique of the MBS175824 kit is based on IGFBP1 antibody-IGFBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IGFBP1 elisa kit
Principle of the Assay: The mouse IGFBP-1 ELISA Kit was based on standard sanuwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A26-N272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then fOllowed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP-1 amount of sample captured in plate.

Background: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and 11.3 The gene is organized in four exons and spans 5.9 kb. 4 The IBP-1 gene is a single copy gene, located on chromosome 7.5 The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KOa.

Typical Testing Data/Standard Curve (for reference only) of IGFBP1 elisa kit
Mouse IGFBP-1 PicoKine ELISA Kit standard curve
IGFBP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for IGFBP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,570 Da
NCBI Official Full Name
Insulin-like growth factor-binding protein 1
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 1
NCBI Official Symbol
Igfbp1  [Similar Products]
NCBI Protein Information
insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1 PRECURSOR (IGFBP-1) (IBP-1) (IGF-BINDING PROTEIN 1)
UniProt Protein Name
Insulin-like growth factor-binding protein 1
UniProt Gene Name
Igfbp1  [Similar Products]
UniProt Synonym Gene Names
Igfbp-1; IBP-1; IGF-binding protein 1; IGFBP-1  [Similar Products]
UniProt Entry Name
IBP1_MOUSE
UniProt Comments for IGFBP1
IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; growth factor binding; insulin-like growth factor II binding

Biological Process: regulation of insulin-like growth factor receptor signaling pathway; regulation of cell growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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