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IGFBP3 elisa kit :: Human insulin-like growth factor binding protein 3 ELISA Kit

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Catalog # MBS700339 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $420 +1 FREE 8GB USB
  96-Strip-Wells  /  $600 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,495 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,715 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

insulin-like growth factor binding protein 3 (IGFBP3), ELISA Kit

Also Known As

Human insulin-like growth factors binding protein 3, IGFBP-3 ELISA Kit

Product Synonym Names
Human insulin-like growth factors binding protein 3; IGFBP-3 ELISA Kit; tcag7.703; BP-53; IBP3; IGF-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; insulin-like growth factor binding protein 3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS700339 Testing Data
Sequence Length
291
OMIM
146732
3D Structure
ModBase 3D Structure for P17936
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human IGFBP3. No significant cross-reactivity or interference between Human IGFBP3 and analogues was observed.
Samples
Serum, plasma and tissue homogenates
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
0.195 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IGFBP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIGFBP3purchase
MBS700339 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the insulin-like growth factor binding protein 3 (IGFBP3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP3. The ELISA analytical biochemical technique of the MBS700339 kit is based on IGFBP3 antibody-IGFBP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IGFBP3 elisa kit
Introduction: Insulin-like growth factor binding protein 3, also known as IGFBP3, is a protein which in humans is encoded by the IGFBP3 gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. IGFBP3 protein levels decrease during the progression of prostate cancer from benign prostatic hyperplasia (BPH) to its metastatic form. Although, production of the protein does not cease completely. IGFBP3 is still made (at a lower level) by prostate cancer cells and secreted into the surrounding environment. However, instead of the full length, functional protein, IGFBP3 is found to be cleaved.This prevents IGFBP3 from binding and sequestering IGFs and the growth factors are free to bind the IGF-1R and promote cell survival.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGFBP-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IGFBP-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGFBP-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IGFBP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of IGFBP3 elisa kit
IGFBP3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700339. Click to request current manual
NCBI/Uniprot data below describe general gene information for IGFBP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,674 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 3 isoform b
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 3
NCBI Official Symbol
IGFBP3  [Similar Products]
NCBI Official Synonym Symbols
IBP3; BP-53
  [Similar Products]
NCBI Protein Information
insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex
UniProt Protein Name
Insulin-like growth factor-binding protein 3
UniProt Gene Name
IGFBP3  [Similar Products]
UniProt Synonym Gene Names
IBP3; IBP-3; IGF-binding protein 3; IGFBP-3  [Similar Products]
UniProt Entry Name
IBP3_HUMAN
NCBI Summary for IGFBP3
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for IGFBP3
Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.1 PublicationManual assertion based on experiment in:Ref.17
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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