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IGFBP3 elisa kit :: Monkey Insulin Like Growth Factor Binding Protein 3 ELISA Kit

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Catalog # MBS738422
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Insulin Like Growth Factor Binding Protein 3 (IGFBP3), ELISA Kit

Popular Item
Also Known As

Monkey Insulin Like Growth Factor Binding Protein 3 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of IGFBP-3. No significant cross-reactivity or interference between IGFBP-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGFBP-3 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IGFBP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIGFBP3purchase
MBS738422 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP3. The ELISA analytical biochemical technique of the MBS738422 kit is based on IGFBP3 antibody-IGFBP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IGFBP3 elisa kit
Intended Uses: This IGFBP-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IGFBP-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||IGFBP-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGFBP-3 antibody and an IGFBP-3-HRP conjugate. The assay sample and buffer are incubated together with IGFBP-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP-3 concentration since IGFBP-3 from samples and IGFBP-3-HRP conjugate compete for the anti-IGFBP-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP-3 from the sample, fewer sites are left to bind IGFBP-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP-3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IGFBP3 elisa kit

Typical Testing Data/Standard Curve of IGFBP3 elisa kit
IGFBP3 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS738422. Click to request current manual
NCBI/Uniprot data below describe general gene information for IGFBP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
31,680 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 3
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 3
NCBI Official Symbol
Igfbp3  [Similar Products]
NCBI Official Synonym Symbols
IGF-BP3
  [Similar Products]
NCBI Protein Information
insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; insulin-like growth factor-binding protein (IGF-BP3)
UniProt Protein Name
Insulin-like growth factor-binding protein 3
UniProt Gene Name
Igfbp3  [Similar Products]
UniProt Synonym Gene Names
Igfbp-3; IBP-3; IGF-binding protein 3; IGFBP-3  [Similar Products]
UniProt Entry Name
IBP3_RAT
NCBI Summary for IGFBP3
an insulin growth factor (Igf) binding protein; involved in modulating IGF-I action [RGD, Feb 2006]
UniProt Comments for IGFBP3
IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation

Cellular Component: extracellular space; nucleus

Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity

Biological Process: positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; regulation of insulin-like growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of smooth muscle cell proliferation; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; osteoblast differentiation; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of MAPKKK cascade; regulation of growth; regulation of cell growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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