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Ihh elisa kit :: Mouse Indian hedgehog protein ELISA Kit

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Catalog # MBS2886533
Unit / Price
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  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Indian hedgehog protein (Ihh), ELISA Kit

Popular Item
Full Product Name

Mouse Indian hedgehog protein ELISA Kit

Product Synonym Names
Indian hedgehog protein; IHH; HHG-2; Ihh
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2886533 Testing Data
3D Structure
ModBase 3D Structure for P97812
Species Reactivity
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Ihh elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Ihhpurchase
MBS2886533 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Indian hedgehog protein (Ihh) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ihh. The ELISA analytical biochemical technique of the MBS2886533 kit is based on Ihh antibody-Ihh antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ihh antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ihh. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Ihh elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Ihh elisa kit
Ihh elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2886533. Click to request current manual
NCBI/Uniprot data below describe general gene information for Ihh. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,485 Da
NCBI Official Full Name
indian hedgehog protein isoform 1 preproprotein
NCBI Official Synonym Full Names
Indian hedgehog
NCBI Official Symbol
NCBI Official Synonym Symbols
HHG-2
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NCBI Protein Information
indian hedgehog protein
UniProt Protein Name
Indian hedgehog protein
UniProt Synonym Protein Names
HHG-2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
IHH_MOUSE
UniProt Comments for Ihh
IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family.

Cellular Component: extracellular region; membrane; plasma membrane; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; hydrolase activity; metal ion binding; patched binding; peptidase activity; protein binding

Biological Process: bone resorption; camera-type eye development; cartilage development; cell communication; cell differentiation; cell fate specification; cell maturation; cell-cell signaling; embryonic camera-type eye morphogenesis; embryonic development; embryonic digestive tract morphogenesis; embryonic digit morphogenesis; embryonic pattern specification; heart looping; in utero embryonic development; intein-mediated protein splicing; morphogenesis of a branching structure; multicellular organism growth; multicellular organismal development; negative regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of eye pigmentation; negative regulation of immature T cell proliferation in the thymus; negative regulation of signal transduction; negative regulation of T cell differentiation in the thymus; neuron development; ossification; osteoblast differentiation; pancreas development; pattern specification process; patterning of blood vessels; positive regulation of alpha-beta T cell differentiation; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of collagen biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; positive regulation of T cell differentiation in the thymus; positive regulation of transcription from RNA polymerase II promoter; proteoglycan metabolic process; proteolysis; regulation of cell differentiation; regulation of growth; skeletal development; smooth muscle development; smoothened signaling pathway; vasculature development; vitelline membrane formation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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