View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List IHH elisa kit :: Rat Hedgehog Homolog, Indian (IHH) ELISA Kit Catalog # MBS453708 Unit / Price 48 Tests / $495 +1 FREE 8GB USB 96 Tests / $655 +1 FREE 8GB USB 5x96 Tests / $2,960 +3 FREE 8GB USB 10x96 Tests / $5,795 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Hedgehog Homolog, Indian (IHH), ELISA Kit Popular Item Full Product Name Rat Hedgehog Homolog, Indian (IHH) ELISA Kit Product Synonym Names BDA1; HHG2 Product Gene Name IHH elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual 3D Structure ModBase 3D Structure for F1LP42 Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of IHH. No significant cross-reactivity or interference between IHH and analogues was observed. Samples Rat tissue homogenates, cell lysates or other biological fluids Assay Type Quantitative Sandwich Detection Range 0.156-10ng/mL Sensitivity < 0.054ng/mL Intra-assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IHH were tested 20 times on one plate, respectively. Intra-Assay: CV<10% Inter-assay Precision Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IHH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12% Preparation and Storage For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of IHH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for IHH purchase MBS453708 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hedgehog Homolog, Indian (IHH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IHH. The ELISA analytical biochemical technique of the MBS453708 kit is based on IHH antibody-IHH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IHH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IHH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for IHH elisa kit Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IHH in rat tissue homogenates, cell lysates or other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IHH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IHH in the samples is then determined by comparing the O.D. of the samples to the standard curve. Typical Testing Data/Standard Curve (for reference only) of IHH elisa kit TOP Sample Manual Insert of MBS453708. NCBI/Uniprot data below describe general gene information for IHH. It may not necessarily be applicable to this product. NCBI GI # 213688398 NCBI GeneID 84399 NCBI Accession # NP_445836.1 [Other Products] NCBI GenBank Nucleotide # NM_053384.1 [Other Products] UniProt Primary Accession # F1LP42 [Other Products] Molecular Weight 45,473 Da TOP NCBI Official Full Name indian hedgehog protein NCBI Official Synonym Full Names Indian hedgehog NCBI Official Symbol Ihh [Similar Products] NCBI Protein Information indian hedgehog protein UniProt Protein Name Hedgehog protein Protein Family Indian hedgehog protein UniProt Gene Name Ihh [Similar Products] TOP NCBI Summary for IHH member of the hedgehog family of secreted morphogens; regulates chondrocyte (cartilage cell) differentiation [RGD, Feb 2006] TOP UniProt Comments for IHH Intercellular signal essential for a variety of patterning events during development. Research Articles on IHH 1. IHH signaling pathway is active during the tendon-bone healing process and appears to be mechanosensitive TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with IHH elisa kit Reference Product PubMed Publications Gli1 elisa kit >109 publications with IHH and Gli1 Ptch1 elisa kit >87 publications with IHH and Ptch1 Smo elisa kit >76 publications with IHH and Smo Gli2 elisa kit >53 publications with IHH and Gli2 Gli3 elisa kit >47 publications with IHH and Gli3 Hhip elisa kit >18 publications with IHH and Hhip Ptch2 elisa kit >7 publications with IHH and Ptch2 Boc elisa kit >3 publications with IHH and Boc Gas1 elisa kit >3 publications with IHH and Gas1 Cdon elisa kit >2 publications with IHH and Cdon Pathways associated with IHH elisa kit Products by Pathway Pathway Diagram Endochondral Ossification Pathway antibodies Endochondral Ossification Pathway Diagram Hedgehog Signaling Pathway antibodies Hedgehog Signaling Pathway Diagram Hedgehog Signaling Pathway antibodies Hedgehog Signaling Pathway Diagram Hedgehog Signaling Pathway antibodies Hedgehog Signaling Pathway Diagram Hedgehog Signaling Pathway antibodies Hedgehog Signaling Pathway Diagram Hedgehog Signaling Pathway antibodies Hedgehog Signaling Pathway Diagram Proteoglycans In Cancer Pathway antibodies Proteoglycans In Cancer Pathway Diagram Proteoglycans In Cancer Pathway antibodies Proteoglycans In Cancer Pathway Diagram Diseases associated with IHH elisa kit Disease Name Pubmed Publications Congenital Abnormalities Antibodies >147 publications with IHH and Congenital Abnormalities Hypertrophy Antibodies >109 publications with IHH and Hypertrophy Infertility, Female Antibodies >32 publications with IHH and Infertility, Female Liver Diseases Antibodies >31 publications with IHH and Liver Diseases Brain Diseases Antibodies >27 publications with IHH and Brain Diseases Cardiovascular Diseases Antibodies >27 publications with IHH and Cardiovascular Diseases Brachydactyly type A1 Antibodies >20 publications with IHH and Brachydactyly type A1 Fibrosis Antibodies >20 publications with IHH and Fibrosis Inflammation Antibodies >15 publications with IHH and Inflammation Liver Neoplasms Antibodies >15 publications with IHH and Liver Neoplasms Organs/Tissues associated with IHH elisa kit Organ/Tissue Name Pubmed Publications Connective Tissue Antibodies >161 publications with IHH and Connective Tissue Embryonic Tissue Antibodies >149 publications with IHH and Embryonic Tissue TOP © 2006-24 MyBioSource.com. 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