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anti-IKBA antibody :: IkappaB-alpha Antibody

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Catalog # MBS859543
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

IkappaB-alpha (IKBA), Antibody

Full Product Name

IkappaB-alpha (Phospho-Ser32/Ser36) Antibody

Product Synonym Names
I-kappa-B-alpha; IkappaBalpha; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
164008
3D Structure
ModBase 3D Structure for P25963
Species Reactivity
Human, Mouse, Rat
Specificity
IkappaB-alpha (Phospho-Ser32/Ser36) antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at serine 32 and serine 36.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human IkappaB-alpha around the phosphorylation site of serine 32 and serine 36 (H-D-SP-G-L-D-SP-M-K).
All Sites
Human: Ser32+Ser36
Mouse: Ser32+Ser36
Rat: Ser32+Ser36
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-IKBA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IKBA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-IKBA antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000

Testing Data of anti-IKBA antibody
P-peptide-+
anti-IKBA antibody Testing Data image
Immunohistochemistry (IHC) of anti-IKBA antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-alpha (Phospho-Ser32/Ser36) antibody. Western blot analysis of extracts from COS-7 cells, using IkappaB-alpha (Phospho-Ser32/Ser36) antibody.
anti-IKBA antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-IKBA antibody
P-peptide-+ Immunofluorescence analysis of LOVO cells, using IkappaB-alpha (Phospho-Ser32/Ser36) antibody.
anti-IKBA antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for IKBA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,609 Da
NCBI Official Full Name
NF-kappa-B inhibitor alpha
NCBI Official Synonym Full Names
NFKB inhibitor alpha
NCBI Official Symbol
NFKBIA  [Similar Products]
NCBI Official Synonym Symbols
IKBA; MAD-3; NFKBI
  [Similar Products]
NCBI Protein Information
NF-kappa-B inhibitor alpha
UniProt Protein Name
NF-kappa-B inhibitor alpha
UniProt Synonym Protein Names
I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
UniProt Gene Name
NFKBIA  [Similar Products]
UniProt Synonym Gene Names
IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha  [Similar Products]
NCBI Summary for IKBA
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
UniProt Comments for IKBA
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Product References and Citations for anti-IKBA antibody
Haskill S., Cell 65:1281-1289(1991). Jungnickel B., J. Exp. Med. 191:395-402(2000). Liu B., Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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