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IL-18BP elisa kit :: Rat Interleukin-18 binding prorein (IL-18BP) ELISA Kit

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Catalog # MBS269372
Unit / Price
  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Interleukin-18 binding prorein (IL-18BP), ELISA Kit

Popular Item
Also Known As

Rat Interleukin-18 binding prorein (IL-18BP) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
199
OMIM
604113
Species Reactivity
Samples
Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-18BP concentration
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-18BP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-18BPpurchase
MBS269372 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-18 binding prorein (IL-18BP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-18BP. The ELISA analytical biochemical technique of the MBS269372 kit is based on IL-18BP antibody-IL-18BP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-18BP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-18BP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-18BP elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat IL-18BP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of IL-18BP elisa kit
IL-18BP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS269372. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-18BP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,478 Da
NCBI Official Full Name
interleukin-18-binding protein isoform c
NCBI Official Synonym Full Names
interleukin 18 binding protein
NCBI Official Symbol
IL18BP  [Similar Products]
NCBI Official Synonym Symbols
IL18BPa
  [Similar Products]
NCBI Protein Information
interleukin-18-binding protein; IL-18BP; tadekinig-alfa; MC51L-53L-54L homolog gene product
UniProt Protein Name
Interleukin-18-binding protein
UniProt Synonym Protein Names
Tadekinig-alfa
UniProt Gene Name
IL18BP  [Similar Products]
UniProt Synonym Gene Names
IL-18BP  [Similar Products]
UniProt Entry Name
I18BP_HUMAN
NCBI Summary for IL-18BP
The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
UniProt Comments for IL-18BP
IL18BP: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; extracellular region

Molecular Function: receptor antagonist activity; interleukin-18 binding

Biological Process: T-helper 1 type immune response; response to lipopolysaccharide
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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