• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IL-1alpha elisa kit :: Rat IL-1alpha ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2505358 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  48-Strip-Wells  /  $310 +1 FREE 8GB USB
  96-Strip-Wells  /  $355 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
  10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-1alpha, ELISA Kit

Popular Item
Also Known As

Rat IL-1alpha (Interleukin 1 Alpha) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
271
OMIM
147760
3D Structure
ModBase 3D Structure for P01583
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat IL-1alpha. No significant cross-reactivity or interference between Rat IL-1alpha and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat IL-1alpha and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-1alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-1alphapurchase
MBS2505358 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-1alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-1alpha. The ELISA analytical biochemical technique of the MBS2505358 kit is based on IL-1alpha antibody-IL-1alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-1alpha elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-1alpha in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1alpha. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IL-1alpha. You can calculate the concentration of Rat IL-1alpha in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of IL-1alpha elisa kit
IL-1alpha elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2505358. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-1alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,607 Da
NCBI Official Full Name
interleukin-1 alpha proprotein
NCBI Official Synonym Full Names
interleukin 1, alpha
NCBI Official Symbol
NCBI Official Synonym Symbols
IL1; IL-1A; IL1F1; IL1-ALPHA
  [Similar Products]
NCBI Protein Information
interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
UniProt Protein Name
Interleukin-1 alpha
UniProt Synonym Protein Names
Hematopoietin-1
UniProt Gene Name
UniProt Synonym Gene Names
IL1F1; IL-1 alpha  [Similar Products]
UniProt Entry Name
IL1A_HUMAN
NCBI Summary for IL-1alpha
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
UniProt Comments for IL-1alpha
IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; interleukin-1 receptor binding; copper ion binding; cytokine activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; negative regulation of cell proliferation; cell proliferation; connective tissue replacement during inflammatory response; positive regulation of angiogenesis; response to copper ion; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of cytokine secretion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000