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IL-1B elisa kit :: Chicken Interleukin 1beta ELISA Kit

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Catalog # MBS282730
Unit / Price
  48-Strip-Wells  /  $390 +1 FREE 8GB USB
  96-Strip-Wells  /  $645 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,940 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,540 +6 FREE 8GB USB
IL-1B elisa kit
Product Name

Interleukin 1beta (IL-1B), ELISA Kit

Popular Item
Also Known As

Chicken Interleukin 1beta (IL-1B) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
269
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Chicken IL-1B. No significant cross-reactivity or interference between Chicken IL-1B and analogues was observed.
Samples
serum, plasma and other biological fluids
Detection Range
1 pg/mL - 200 pg/mL
Sensitivity
0.4 pg/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-1B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-1Bpurchase
MBS282730 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 1beta (IL-1B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-1B. The ELISA analytical biochemical technique of the MBS282730 kit is based on IL-1B antibody-IL-1B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-1B elisa kit
Intended Uses: For the quantitative detection of Chicken Interleukin 1? (IL-1B) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IL-1B and Horseradish Peroxidase (HRP) conjugated IL-1B. The competitive inhibition reaction is launched between with HRP labeled IL-1B and unlabeled IL-1B with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-1B in the sample. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS282730. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-1B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,931 Da
NCBI Official Full Name
interleukin-1 beta
NCBI Official Synonym Full Names
interleukin 1 beta
NCBI Official Symbol
NCBI Official Synonym Symbols
Il-1b; IL-1beta
  [Similar Products]
NCBI Protein Information
interleukin-1 beta; IL-1 beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
UniProt Synonym Gene Names
IL-1 beta  [Similar Products]
UniProt Entry Name
IL1B_MOUSE
NCBI Summary for IL-1B
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]
UniProt Comments for IL-1B
IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.

Protein type: Cytokine

Cellular Component: extracellular space; cell; extracellular region; intracellular; cytosol; vesicle; secretory granule

Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity; receptor binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of MAP kinase activity; positive regulation of JNK activity; positive regulation of nitric oxide biosynthetic process; negative regulation of glutamate secretion; activation of MAPK activity; positive regulation of apoptosis; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; response to lipopolysaccharide; positive regulation of glial cell differentiation; positive regulation of lipid catabolic process; positive regulation of NF-kappaB import into nucleus; fever; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; neutrophil chemotaxis; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; positive regulation of interleukin-6 production; interleukin-1 beta production; social behavior; positive regulation of angiogenesis; negative regulation of neuron differentiation; positive regulation of cell division; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of lipid metabolic process; leukocyte migration; sequestering of triacylglycerol; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; memory; response to ATP; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of fever; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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