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IL-1beta elisa kit :: Chicken Interleukin 1beta, IL-1beta ELISA Kit

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Catalog # MBS703082
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $475 +1 FREE 8GB USB
  96-Strip-Wells  /  $675 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,140 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Interleukin 1beta, IL-1beta, ELISA Kit

Popular Item
Full Product Name

Chicken Interleukin 1beta, IL-1beta ELISA Kit

Product Synonym Names
Chicken Interleukin 1beta; IL-1beta ELISA Kit; Interleukin 1beta; IL-1beta
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703082 Testing Data
OMIM
147720
3D Structure
ModBase 3D Structure for P01584
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of chicken IL-1beta. No significant cross-reactivity or interference between chicken IL-1beta and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Competitive
Detection Range
0. 27 pg/ml -200 pg/ml.
Sensitivity
The minimum detectable dose of chicken IL-1beta is typically less than 0.27 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest chicken IL-1beta concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-1beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-1betapurchase
MBS703082 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 1beta, IL-1beta, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-1beta. The ELISA analytical biochemical technique of the MBS703082 kit is based on IL-1beta antibody-IL-1beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-1beta elisa kit
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IL-1beta. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody specific to IL-1beta. The competitive inhibition reaction is launched between with pre-coated IL-1beta and IL-1beta in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-1beta in the sample. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of IL-1beta elisa kit
IL-1beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703082. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-1beta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,748 Da
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1, beta
NCBI Official Symbol
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
  [Similar Products]
NCBI Protein Information
interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
UniProt Protein Name
Interleukin-1 beta
UniProt Synonym Protein Names
Catabolin
UniProt Gene Name
UniProt Synonym Gene Names
IL1F2; IL-1 beta  [Similar Products]
UniProt Entry Name
IL1B_HUMAN
NCBI Summary for IL-1beta
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
UniProt Comments for IL-1beta
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.1 PublicationManual assertion based on experiment in:Ref.15
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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