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IL-1beta elisa kit :: Mouse IL-1beta ELISA Kit

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Catalog # MBS355354
Unit / Price
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  96 Tests  /  $440 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-1beta, ELISA Kit

Popular Item
Full Product Name

IL-1beta Mouse ELISA Kit

Product Synonym Names
Mouse IL-1beta
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 2; NC_000068.7 (129364579..129371120, complement). Location: 2 F; 2 62.97 cM
3D Structure
ModBase 3D Structure for P10749
Species Reactivity
Assay Type
Sandwich
Detection Range
5 pg/ml-100 pg/ml
Preparation and Storage
Store at 2-8 degree C for 6 months.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-1beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IL-1betapurchase
MBS355354 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-1beta, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-1beta. The ELISA analytical biochemical technique of the MBS355354 kit is based on IL-1beta antibody-IL-1beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-1beta elisa kit

Principle of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-IL-1betaantibody was pre-coated onto 96-well plates. And the HRP conjugated anti-IL-1beta antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1beta can be calculated.

Background: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. It can reset glucose homeostasis at central levels. It cause oligodendrocyte death in coculture with astrocytes and microglia, and also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Pathak et al. (2011) proposed that IL1B blockade may be a viable therapy for patients with autoimmune inner ear disease or sensorineural hearing loss that fail to respond to corticosteroids.
Applications Tested/Suitable for IL-1beta elisa kit
Quantitative sELISA (EIA)
Application Notes for IL-1beta elisa kit
For quantitative detection of IL-1beta in mouse serum, plasma, urine, cell culture supernatant or tissue samples.

Typical Testing Data/Standard Curve (for reference only) of IL-1beta elisa kit
IL-1beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS355354. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-1beta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
30,931 Da
NCBI Official Full Name
interleukin-1 beta
NCBI Official Synonym Full Names
interleukin 1 beta
NCBI Official Symbol
NCBI Official Synonym Symbols
Il-1b; IL-1beta
  [Similar Products]
NCBI Protein Information
interleukin-1 beta; IL-1 beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
UniProt Synonym Gene Names
IL-1 beta  [Similar Products]
UniProt Entry Name
IL1B_MOUSE
NCBI Summary for IL-1beta
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]
UniProt Comments for IL-1beta
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Subunit structure: Monomer.

Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.

Sequence similarities: Belongs to the IL-1 family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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