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IL 1RA recombinant protein :: Interleukin-1 Receptor Antagonist Recombinant Protein

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Catalog # MBS142080
Unit / Price
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  0.02 mg  /  $140 +1 FREE 8GB USB
  0.1 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $1,030 +1 FREE 8GB USB
IL 1RA recombinant protein
Product Name

Interleukin-1 Receptor Antagonist (IL 1RA), Recombinant Protein

Full Product Name

Recombinant Human Interleukin-1 Receptor Antagonist

Product Synonym Names
IL 1RA Human; Interleukin-1 Receptor Antagonist Human Recombinant; IRAP; IL1F3; IL1RA; IL-1ra3; ICIL-1RA; IL1RN; IL1 inhibitor; IL-1ra; MGC10430
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pr o-Ser-Gly
OMIM
147679
3D Structure
ModBase 3D Structure for P18510
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
Lyophilized protein with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-1 ra in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1 ra as a Reference Standard.
Biological Activity
The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml corresponding to a Specific Activity of 2,000,000IU/mg.
Preparation and Storage
Lyophilized Interleukin 1ra although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL-1ra should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Other Notes
Small volumes of IL 1RA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IL 1RA recombinant protein
Description: IL1ra Human Recombinant produced in E Coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17000 Dalton. The IL1ra is purified by proprietary chromatographic techniques.

Introduction: Interleukin-1 ra is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
NCBI/Uniprot data below describe general gene information for IL 1RA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,142 Da
NCBI Official Full Name
interleukin-1 receptor antagonist protein isoform 3
NCBI Official Synonym Full Names
interleukin 1 receptor antagonist
NCBI Official Symbol
IL1RN  [Similar Products]
NCBI Official Synonym Symbols
DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
  [Similar Products]
NCBI Protein Information
interleukin-1 receptor antagonist protein; IL1 inhibitor; IL1RN (IL1F3); intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); type II interleukin-1 receptor antagonist
UniProt Protein Name
Interleukin-1 receptor antagonist protein
UniProt Synonym Protein Names
ICIL-1RA; IL1 inhibitor; INN: Anakinra
UniProt Gene Name
IL1RN  [Similar Products]
UniProt Synonym Gene Names
IL1F3; IL1RA; IL-1RN; IL-1ra; IRAP  [Similar Products]
UniProt Entry Name
IL1RA_HUMAN
NCBI Summary for IL 1RA
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Comments for IL 1RA
IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Vesicle; Secreted

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: extracellular space; cytoplasm; plasma membrane; intracellular

Molecular Function: interleukin-1 Type I receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type II receptor binding; cytokine activity; interleukin-1, Type I receptor binding; interleukin-1 receptor antagonist activity

Biological Process: response to drug; positive regulation of JNK activity; negative regulation of heterotypic cell-cell adhesion; negative regulation of glutamate secretion; response to glucocorticoid stimulus; carboxylic acid metabolic process; response to lipopolysaccharide; female pregnancy; fever; sensory perception of pain; chronic inflammatory response to antigenic stimulus; memory; response to starvation; insulin secretion; acute-phase response; immune response; negative regulation of cytokine and chemokine mediated signaling pathway; lipid metabolic process; response to activity; negative regulation of membrane potential; negative regulation of cell migration; negative regulation of apoptosis

Disease: Gastric Cancer, Hereditary Diffuse; Microvascular Complications Of Diabetes, Susceptibility To, 4; Osteomyelitis, Sterile Multifocal, With Periostitis And Pustulosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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