• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IL-21 elisa kit :: Human IL-21 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2503448
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-21, ELISA Kit

Popular Item
Full Product Name

Human IL-21 (Interleukin 21) ELISA Kit

Product Gene Name

IL-21 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
TOP
Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
Request Current Manual
OMIM
605384
3D Structure
ModBase 3D Structure for Q9HBE4
Species Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human IL-21. No significant cross-reactivity or interference between Human IL-21 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human IL-21 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-21 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-21purchase
MBS2503448 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-21, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-21. The ELISA analytical biochemical technique of the MBS2503448 kit is based on IL-21 antibody-IL-21 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-21 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-21. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
TOP
Related Product Information for
IL-21 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-21 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-21. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human IL-21 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IL-21. You can calculate the concentration of Human IL-21 in the samples by comparing the OD of the samples to the standard curve.
Typical Testing Data/Standard Curve (for reference only) of IL-21 elisa kit
IL-21 elisa kit Typical Testing Data/Standard Curve (for reference only) image
TOP
Sample Manual Insert of MBS2503448. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-21. It may not necessarily be applicable to this product.
NCBI GI #
333033767
NCBI GeneID
59067
NCBI Accession #
NP_001193935.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001207006.2 [Other Products]
UniProt Primary Accession #
Q9HBE4 [Other Products]
UniProt Secondary Accession #
A5J0L4[Other Products]
UniProt Related Accession #
Q9HBE4[Other Products]
Molecular Weight
16,867 Da
TOP
NCBI Official Full Name
interleukin-21 isoform 2
NCBI Official Synonym Full Names
interleukin 21
NCBI Official Symbol
IL21  [Similar Products]
NCBI Official Synonym Symbols
Za11; IL-21; CVID11
  [Similar Products]
NCBI Protein Information
interleukin-21; interleukin-21 isoform
UniProt Protein Name
Interleukin-21
UniProt Synonym Protein Names
Za11
UniProt Gene Name
IL21  [Similar Products]
UniProt Synonym Gene Names
IL-21  [Similar Products]
UniProt Entry Name
IL21_HUMAN
TOP
NCBI Summary for IL-21
This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
TOP
UniProt Comments for IL-21
IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Apoptosis; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; signal transduction; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tissue remodeling; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; immune response; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response

Disease: Immunodeficiency, Common Variable, 11
Research Articles on IL-21
1. IL-21 signals through the STAT-3 and STAT- 5b signaling pathway in the CIK cell pool.
TOP
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
TOP
Products associated withIL-21 elisa kit
 Reference Product  PubMed Publications
 IL21R elisa kit  >372 publications with IL-21 and IL21R
 STAT3 elisa kit  >118 publications with IL-21 and STAT3
 IL15 elisa kit  >83 publications with IL-21 and IL15
 IL4 elisa kit  >14 publications with IL-21 and IL4
 IL13 elisa kit  >6 publications with IL-21 and IL13
 IL9 elisa kit  >5 publications with IL-21 and IL9
 IL7 elisa kit  >4 publications with IL-21 and IL7
 NFATC2 elisa kit  >1 publications with IL-21 and NFATC2
 TSLP elisa kit  >1 publications with IL-21 and TSLP
Pathways associated with IL-21 elisa kit
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
Diseases associated with IL-21 elisa kit
 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >270 publications with IL-21 and Autoimmune Diseases
 Inflammation Antibodies  >175 publications with IL-21 and Inflammation
 Disease Models, Animal Antibodies  >119 publications with IL-21 and Disease Models, Animal
 Lupus Erythematosus, Systemic Antibodies  >73 publications with IL-21 and Lupus Erythematosus, Systemic
 Necrosis Antibodies  >64 publications with IL-21 and Necrosis
 Liver Diseases Antibodies  >45 publications with IL-21 and Liver Diseases
 Celiac Disease Antibodies  >32 publications with IL-21 and Celiac Disease
 Hypersensitivity Antibodies  >29 publications with IL-21 and Hypersensitivity
 Kidney Diseases Antibodies  >28 publications with IL-21 and Kidney Diseases
 Heart Diseases Antibodies  >16 publications with IL-21 and Heart Diseases
Organs/Tissues associated with IL-21 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with IL-21 and Uncharacterized Tissue
TOP
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000