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IL-25 elisa kit :: Rat IL-25 ELISA Kit

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Catalog # MBS2515787
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-25, ELISA Kit

Popular Item
Full Product Name

Rat IL-25 (Interleukin 25) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605658
Species Reactivity
Specificity
This kit recognizes natural and recombinant Rat IL-25. No significant cross-reactivity or interference between Rat IL-25 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Rat IL-25 concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-25 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IL-25purchase
MBS2515787 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-25, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-25. The ELISA analytical biochemical technique of the MBS2515787 kit is based on IL-25 antibody-IL-25 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-25 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-25. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-25 elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-25. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-25 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-25. You can calculate the concentration of IL-25 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of IL-25 elisa kit
IL-25 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2515787. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-25. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,523 Da
NCBI Official Full Name
interleukin-25 isoform 2
NCBI Official Synonym Full Names
interleukin 25
NCBI Official Symbol
NCBI Official Synonym Symbols
IL17E
  [Similar Products]
NCBI Protein Information
interleukin-25; interleukin-17E
UniProt Protein Name
Interleukin-25
UniProt Synonym Protein Names
Interleukin-17E; IL-17E
UniProt Gene Name
UniProt Synonym Gene Names
IL17E; IL-25; IL-17E  [Similar Products]
UniProt Entry Name
IL25_HUMAN
NCBI Summary for IL-25
The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
UniProt Comments for IL-25
IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-17E receptor binding

Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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