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IL-2Rbeta elisa kit :: Rat IL-2Rbeta ELISA Kit

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Catalog # MBS2514539
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-2Rbeta, ELISA Kit

Popular Item
Full Product Name

Rat IL-2Rbeta (Interleukin-2 Receptor-beta) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P16297
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat IL-2Rbeta. No significant cross-reactivity or interference between Rat IL-2Rbeta and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat IL-2Rbeta and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-2Rbeta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-2Rbetapurchase
MBS2514539 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-2Rbeta, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-2Rbeta. The ELISA analytical biochemical technique of the MBS2514539 kit is based on IL-2Rbeta antibody-IL-2Rbeta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-2Rbeta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-2Rbeta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-2Rbeta elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-2Rbeta in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2Rbeta. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2Rbeta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-2Rbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IL-2Rbeta. You can calculate the concentration of Rat IL-2Rbeta in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of IL-2Rbeta elisa kit
IL-2Rbeta elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2514539. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-2Rbeta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60,539 Da
NCBI Official Full Name
interleukin-2 receptor subunit beta
NCBI Official Synonym Full Names
interleukin 2 receptor, beta chain
NCBI Official Symbol
Il2rb  [Similar Products]
NCBI Official Synonym Symbols
p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
  [Similar Products]
NCBI Protein Information
interleukin-2 receptor subunit beta; IL-15 receptor beta chain; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; IL-2R subunit beta; IL-2RB; high affinity IL-2 receptor subunit beta; p70-75
UniProt Protein Name
Interleukin-2 receptor subunit beta
UniProt Synonym Protein Names
High affinity IL-2 receptor subunit beta; p70-75; CD_antigen: CD122
UniProt Gene Name
Il2rb  [Similar Products]
UniProt Synonym Gene Names
IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB  [Similar Products]
UniProt Entry Name
IL2RB_MOUSE
NCBI Summary for IL-2Rbeta
The interleukin 2 receptor is composed of alpha and beta subunits. The beta subunit encoded by this gene is very homologous to the human beta subunit and also shows structural similarity to other cytokine receptors. [provided by RefSeq, Jul 2008]
UniProt Comments for IL-2Rbeta
IL2RB: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Interacts with SHB upon interleukin stimulation. Interacts with HTLV-1 accessory protein p12I. Belongs to the type I cytokine receptor family. Type 4 subfamily.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-2 receptor activity; interleukin-2 binding

Biological Process: cytokine and chemokine mediated signaling pathway; natural killer cell activation; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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