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IL-4 elisa kit :: Canine IL-4 ELISA Kit

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Catalog # MBS2516257
Unit / Price
  48-Strip-Wells  /  $490 +1 FREE 8GB USB
  96-Strip-Wells  /  $585 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,475 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,360 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name


Popular Item
Also Known As

Canine IL-4 (Interleukin 4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2516257 COA
Sequence Length
Species Reactivity
This kit recognizes natural and some recombinant Canine IL-4. No Significant cross -reactivity or interference between Canine IL-4and analogues was observed.
Serum, Plasma, Biological Fluids
Assay Type
Detection Range
Min: 9.375pg/mL; Max: 1000pg/mL
Intra-assay Precision
Intra -assay Precision (Precision within an assay): 3 samples with low, midrange and high level CanineIL-4 were tested 20times on one plate, respectively.
Inter-assay Precision
Inter -assay Precision (Precision between assays): 3 samples with low, midrange and high level CanineIL-4 were testedon 3 different plates, 20replicates in each plate.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-4purchase
MBS2516257 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-4. The ELISA analytical biochemical technique of the MBS2516257 kit is based on IL-4 antibody-IL-4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-4 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of CanineIL-4concentrations in serum, plasma and other biological fluids.

Typical Testing Data/Standard Curve (for reference only) of IL-4 elisa kit
IL-4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2516257. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-4. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,797 Da
NCBI Official Full Name
NCBI Official Synonym Full Names
interleukin 4
NCBI Official Symbol
NCBI Official Synonym Symbols
BSF1; IL-4; BCGF1; BSF-1; BCGF-1
  [Similar Products]
NCBI Protein Information
interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; interleukin 4 variant 2; lymphocyte stimulatory factor 1; pitrakinra
UniProt Protein Name
UniProt Synonym Protein Names
B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
UniProt Gene Name
UniProt Synonym Gene Names
IL-4; BSF-1  [Similar Products]
UniProt Entry Name
NCBI Summary for IL-4
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Comments for IL-4
IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: regulation of isotype switching; positive regulation of isotype switching to IgG isotypes; negative regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; female pregnancy; positive regulation of activated T cell proliferation; chemotaxis; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; connective tissue growth factor biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; T-helper 2 type immune response; regulation of phosphorylation; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; response to ethanol; positive regulation of chemokine biosynthetic process; innate immune response in mucosa; positive regulation of T cell differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; retina development in camera-type eye; response to cytokine stimulus; B cell differentiation; cellular defense response; immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of apoptosis

Disease: Stroke, Ischemic
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with IL-4 elisa kit
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