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IL-5 elisa kit :: Interleukin-5 ELISA Kit

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Catalog # MBS590010
Unit / Price
  96 Wells  /  $605 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Interleukin-5 (IL-5), ELISA Kit

Full Product Name

Interleukin-5 (IL-5)

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
147850
Specificity
This sandwich ELISA can detect both natural and recombinant human IL-5. This kit exhibits no detectable cross-reactivity with human; GM-CSF, GRO a, GRO b, INF-g, INF a, IL-1a, IL-1b, IL-1 RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL- 11, IL-12, IL-13, IL-15, TNF-a, mouse IL-5 and IL-9.
Samples
Serum, plasma, cell culture medium, and urine
Assay Type
Sandwich
Sensitivity
The minimum detectable quantities of human IL-5 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 7.5 pg/mL and 6.3 pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities.
Intra-assay Precision
To determine within-run precision, three different samples of known concentration were assayed by replicates of 20 in 1 assay.
Inter-assay Precision
To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
Intended Uses
This Human Interleukin 5 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 5 (IL-5) concentrations in serum, plasma, cell culture medium, and urine. This kit is intended for LABORATORY RESEARCH ONLY and is not to be used for diagnostic or therapeutic purposes.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-5purchase
MBS590010 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-5 (IL-5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-5. The ELISA analytical biochemical technique of the MBS590010 kit is based on IL-5 antibody-IL-5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-5 elisa kit
Principle of the assay: This IL-5 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-5 and incubated. IL-5, if present, will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-5 and other components of the sample. In order to quantitatively determine the amount of IL-5 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-5, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.

Typical Testing Data/Standard Curve (for reference only) of IL-5 elisa kit
IL-5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS590010. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,238 Da
NCBI Official Full Name
interleukin-5
NCBI Official Synonym Full Names
interleukin 5
NCBI Official Symbol
NCBI Official Synonym Symbols
EDF; TRF; IL-5
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NCBI Protein Information
interleukin-5; B-cell differentiation factor I; T-cell replacing factor; colony-stimulating factor, eosinophil; eosinophil differentiation factor
UniProt Protein Name
Interleukin-5
UniProt Synonym Protein Names
B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
Protein Family
UniProt Gene Name
UniProt Entry Name
IL5_HUMAN
NCBI Summary for IL-5
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
UniProt Comments for IL-5
IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with IL-5 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >8 publications with IL-5 and Thymus
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