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IL-6 elisa kit :: Rat IL-6 ELISA Kit

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Catalog # MBS355410
Unit / Price
  96 Tests  /  $410 +1 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-6, ELISA Kit

Popular Item
Also Known As

IL-6 Rat ELISA Kit

Product Synonym Names
Rat IL-6
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 4; NC_005103.3 (3095536..3100112). Location: 4q11
3D Structure
ModBase 3D Structure for P20607
Species Reactivity
Samples
rat serum, body fluids, tissue lysates or cell culture supernates
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 5 pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-6purchase
MBS355410 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-6, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-6. The ELISA analytical biochemical technique of the MBS355410 kit is based on IL-6 antibody-IL-6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-6 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-6 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-6 can be calculated.

Background: Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. It acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 is one of the most important mediators of fever and of the acute phase response. It is capable of crossing the blood brain barrier and initiating synthesis of PGE2 in the hypothalamus, thereby changing the body's temperature setpoint. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 are used to treat postmenopausal osteoporosis. IL-6 is relevant to many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, prostate cancer, and rheumatoid arthritis.
Applications Tested/Suitable for IL-6 elisa kit
Quantitative sELISA (EIA)
Application Notes for IL-6 elisa kit
For quantitative detection of IL-6 in rat serum, body fluids, tissue lysates or cell culture supernates.

Typical Testing Data/Standard Curve (for reference only) of IL-6 elisa kit
IL-6 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS355410. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
24,357 Da
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
NCBI Official Synonym Symbols
ILg6; Ifnb2
  [Similar Products]
NCBI Protein Information
interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
UniProt Protein Name
Interleukin-6
UniProt Gene Name
UniProt Synonym Gene Names
Il-6; IL-6  [Similar Products]
UniProt Entry Name
IL6_RAT
NCBI Summary for IL-6
a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
UniProt Comments for IL-6
Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

By similarity.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-6 superfamily.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with IL-6 elisa kit
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