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IL-6R elisa kit :: Canine Interleukin 6 receptor ELISA Kit

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Catalog # MBS744847
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Interleukin 6 receptor (IL-6R), ELISA Kit

Popular Item
Full Product Name

Canine Interleukin 6 receptor ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
614752
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-6R. No significant cross-reactivity or interference between IL-6R and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-6R and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL-6R elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL-6Rpurchase
MBS744847 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 6 receptor (IL-6R) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-6R. The ELISA analytical biochemical technique of the MBS744847 kit is based on IL-6R antibody-IL-6R antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-6R antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-6R. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL-6R elisa kit
Intended Uses: This IL-6R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IL-6R. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||IL-6R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-6R antibody and an IL-6R-HRP conjugate. The assay sample and buffer are incubated together with IL-6R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-6R concentration since IL-6R from samples and IL-6R-HRP conjugate compete for the anti-IL-6R antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-6R from the sample, fewer sites are left to bind IL-6R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6R concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IL-6R elisa kit

Typical Testing Data/Standard Curve of IL-6R elisa kit
IL-6R elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS744847. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL-6R. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,237 Da
NCBI Official Full Name
interleukin-6 receptor subunit alpha isoform 3
NCBI Official Synonym Full Names
interleukin 6 receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
  [Similar Products]
NCBI Protein Information
interleukin-6 receptor subunit alpha; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha
UniProt Protein Name
Interleukin-6 receptor subunit alpha
UniProt Synonym Protein Names
IL-6R 1; Membrane glycoprotein 80; gp80; CD_antigen: CD126
UniProt Gene Name
UniProt Synonym Gene Names
IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80  [Similar Products]
UniProt Entry Name
IL6RA_HUMAN
NCBI Summary for IL-6R
This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]
UniProt Comments for IL-6R
IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; basolateral plasma membrane; apical plasma membrane; extracellular region; plasma membrane; interleukin-6 receptor complex

Molecular Function: protein binding; protein homodimerization activity; interleukin-6 binding; interleukin-6 receptor binding; enzyme binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity

Biological Process: negative regulation of collagen biosynthetic process; cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; neutrophil mediated immunity; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; defense response to Gram-negative bacterium; endocrine pancreas development; positive regulation of chemokine production; positive regulation of tyrosine phosphorylation of Stat3 protein; activation of NF-kappaB transcription factor; monocyte chemotaxis; positive regulation of osteoblast differentiation; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; response to cytokine stimulus; positive regulation of cell proliferation; acute-phase response; negative regulation of interleukin-8 production; hepatic immune response

Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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