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IL10 elisa kit :: Rat IL-10 ELISA Kit

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Catalog # MBS175998
Unit / Price
  96-Strip-Wells  /  $410 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-10 (IL10), ELISA Kit

Popular Item
Also Known As

Rat IL-10 PicoKine ELISA Kit

Product Synonym Names
Interleukin-10; interleukin 10; CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL-10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin-10; Interleukin10; MGC126450; MGC126451; T cell growth inhibitory factor; TGIF
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
178
3D Structure
ModBase 3D Structure for P29456
Species Reactivity
Specificity
Natural and recombinant rat IL-10
Samples
Cell culture supernates, serum and plasma (EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 4pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL10purchase
MBS175998 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-10 (IL10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL10. The ELISA analytical biochemical technique of the MBS175998 kit is based on IL10 antibody-IL10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL10 elisa kit
Principle of the assay: rat IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards (E.coli,S19-N178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-10 amount of sample captured in plate.
Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is animportant cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatorycytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recentlydescribed natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability maycontribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is ofspecial interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to humanchromosome 1.7.

Typical Testing Data/Standard Curve (for reference only) of IL10 elisa kit
Rat IL-10 PicoKine ELISA Kit standard curve
IL10 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175998. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,426 Da
NCBI Official Full Name
Interleukin-10
NCBI Official Synonym Full Names
interleukin 10
NCBI Official Symbol
NCBI Official Synonym Symbols
IL10X
  [Similar Products]
NCBI Protein Information
interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor
UniProt Protein Name
Interleukin-10
UniProt Synonym Protein Names
Cytokine synthesis inhibitory factor; CSIF
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Il-10; IL-10; CSIF  [Similar Products]
UniProt Entry Name
IL10_RAT
NCBI Summary for IL10
factor involved in the inhibition of cytokine synthesis [RGD, Feb 2006]
UniProt Comments for IL10
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with IL10 elisa kit
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