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IL16 elisa kit :: Human IL-16 ELISA Kit

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Catalog # MBS176490
Unit / Price
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  96-Strip-Wells  /  $390 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-16 (IL16), ELISA Kit

Popular Item
Full Product Name

Human IL-16 PicoKine ELISA Kit

Product Synonym Names
Pro-interleukin-16; interleukin 16; FLJ16806; FLJ42735; FLJ44234; HGNC:5980; HsT19289; IL-16; IL16; IL16_HUMAN; Interleukin 16; Interleukin 16 precursor; Interleukin-16; KIAA4048; LCF; Lymphocyte chemoattractant factor; mKIAA4048; Neuronal interleukin 16; NIL16; OTTHUMP00000185571; OTTHUMP00000190849; OTTHUMP00000190850; PrIL16; Pro interleukin 16; Prointerleukin 16
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
603035
3D Structure
ModBase 3D Structure for Q14005
Species Reactivity
Specificity
Natural and recombinant human IL-16
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 1pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL16 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL16purchase
MBS176490 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-16 (IL16) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL16. The ELISA analytical biochemical technique of the MBS176490 kit is based on IL16 antibody-IL16 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL16 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL16. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL16 elisa kit
Principle of the assay: human IL-16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-16 has been precoated onto 96-well plates. Standards(E.coli, P2-S130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-16 amount of sample captured in plate.
Background: Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.

Typical Testing Data/Standard Curve (for reference only) of IL16 elisa kit
IL16 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS176490. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL16. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
66,646 Da
NCBI Official Full Name
Pro-interleukin-16
NCBI Official Synonym Full Names
interleukin 16
NCBI Official Symbol
NCBI Official Synonym Symbols
LCF; NIL16; PRIL16; prIL-16
  [Similar Products]
NCBI Protein Information
pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor
UniProt Protein Name
Pro-interleukin-16
UniProt Synonym Protein Names
Lymphocyte chemoattractant factor; LCF
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IL-16; LCF  [Similar Products]
UniProt Entry Name
IL16_HUMAN
NCBI Summary for IL16
The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
UniProt Comments for IL16
IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: viral reproduction; regulation of transcription, DNA-dependent; transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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