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IL18 elisa kit :: Human Interleukin 18 (IL18) ELISA Kit

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Catalog # MBS2020065 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $245 +1 FREE 8GB USB
  48-Strip-Wells  /  $415 +1 FREE 8GB USB
  96-Strip-Wells  /  $555 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,220 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,000 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Interleukin 18 (IL18), ELISA Kit

Popular Item
Also Known As

Interleukin 18 (IL18) ELISA Kit

Product Synonym Names
IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
193
OMIM
600953
3D Structure
ModBase 3D Structure for Q14116
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 18 (IL18).
No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Urine, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
15.6-1000pg/mL
Sensitivity
< 5.9pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Interleukin 18 (IL18) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Interleukin 18 (IL18) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL18 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL18purchase
MBS2020065 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 18 (IL18) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL18. The ELISA analytical biochemical technique of the MBS2020065 kit is based on IL18 antibody-IL18 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL18 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL18. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL18 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 (IL18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 (IL18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 (IL18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 18 (IL18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for IL18 elisa kit

Typical Testing Data/Standard Curve (for reference only) of IL18 elisa kit
IL18 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020065. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL18. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,896 Da
NCBI Official Full Name
interleukin-18 isoform 1 proprotein
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
NCBI Official Synonym Symbols
IGIF; IL-18; IL-1g; IL1F4
  [Similar Products]
NCBI Protein Information
interleukin-18
UniProt Protein Name
Interleukin-18
UniProt Synonym Protein Names
Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma  [Similar Products]
UniProt Entry Name
IL18_HUMAN
NCBI Summary for IL18
The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
UniProt Comments for IL18
IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; regulation of cell adhesion; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; T-helper 1 type immune response; T-helper 2 type immune response; interleukin-2 biosynthetic process; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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