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IL23A elisa kit :: Human interleukin 23, alpha subunit p19 ELISA Kit

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Catalog # MBS706156
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Typical Testing Data/Standard Curve (for reference only)
Product Name

interleukin 23, alpha subunit p19 (IL23A), ELISA Kit

Popular Item
Full Product Name

Human Interleukin-23 subunit alpha, IL-23A ELISA Kit

Product Synonym Names
Human Interleukin-23 subunit alpha (IL-23A) Elisa kit; IL-23; IL-23A; IL23P19; MGC79388; P19; SGRF; JKA3 induced upon T-cell activation; interleukin 23 p19 subunit; interleukin 23; alpha subunit p19
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS706156 Testing Data
OMIM
605580
3D Structure
ModBase 3D Structure for Q9NPF7
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human IL-23A. No significant cross-reactivity or interference between human IL-23A and analogues was observed.
Samples
serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
<15.6 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL23A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IL23Apurchase
MBS706156 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interleukin 23, alpha subunit p19 (IL23A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL23A. The ELISA analytical biochemical technique of the MBS706156 kit is based on IL23A antibody-IL23A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL23A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL23A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL23A elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-23A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-23A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-23A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-23A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of IL23A elisa kit
IL23A elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for IL23A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,730 Da
NCBI Official Full Name
interleukin-23 subunit alpha
NCBI Official Synonym Full Names
interleukin 23, alpha subunit p19
NCBI Official Symbol
IL23A  [Similar Products]
NCBI Official Synonym Symbols
P19; SGRF; IL-23; IL-23A; IL23P19
  [Similar Products]
NCBI Protein Information
interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor
UniProt Protein Name
Interleukin-23 subunit alpha
UniProt Synonym Protein Names
Interleukin-23 subunit p19; IL-23p19
Protein Family
UniProt Gene Name
IL23A  [Similar Products]
UniProt Synonym Gene Names
SGRF; UNQ2498/PRO5798; IL-23 subunit alpha; IL-23-A; IL-23p19  [Similar Products]
UniProt Entry Name
IL23A_HUMAN
NCBI Summary for IL23A
This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008]
UniProt Comments for IL23A
Function: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.3 PublicationsManual assertion based on experiment in:Ref.1
Research Articles on IL23A
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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