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IL2RA elisa kit :: Dog interleukin 2 receptor, alpha ELISA Kit

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Catalog # MBS944418
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

interleukin 2 receptor, alpha (IL2RA), ELISA Kit

Full Product Name

Dog Interleukin-2 receptor subunit alpha, IL2RA ELISA Kit

Product Synonym Names
Dog Interleukin-2 receptor subunit alpha (IL2RA) ELISA kit; RP11-536K7.1; CD25; IDDM10; IL2R; TCGFR; ; interleukin 2 receptor; alpha
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS944418 Testing Data
3D Structure
ModBase 3D Structure for O62802
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of dog IL2RA. No significant cross-reactivity or interference between dog IL2RA and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
62.5 pg/ml - 4000 pg/ml.
Sensitivity
The minimum detectable dose of dog IL2RA is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL2RA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IL2RApurchase
MBS944418 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interleukin 2 receptor, alpha (IL2RA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL2RA. The ELISA analytical biochemical technique of the MBS944418 kit is based on IL2RA antibody-IL2RA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL2RA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL2RA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL2RA elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL2RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL2RA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL2RA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL2RA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of IL2RA elisa kit
IL2RA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS944418. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL2RA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
30,357 Da
NCBI Official Full Name
interleukin-2 receptor subunit alpha
NCBI Official Symbol
IL2RA  [Similar Products]
NCBI Official Synonym Symbols
CD25; IL-2RA
  [Similar Products]
NCBI Protein Information
interleukin-2 receptor subunit alpha; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; interleukin 2 receptor alpha chain; interleukin-2 receptor alpha subunit
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Synonym Protein Names
TAC antigen; p55; CD_antigen: CD25
Protein Family
UniProt Gene Name
IL2RA  [Similar Products]
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA  [Similar Products]
UniProt Entry Name
IL2RA_CANFA
UniProt Comments for IL2RA
Function: Receptor for interleukin-2.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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