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Il36g elisa kit :: Mouse Interleukin-36 gamma ELISA Kit

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Catalog # MBS288173
Unit / Price
  48-Strip-Wells  /  $305 +1 FREE 8GB USB
  96-Strip-Wells  /  $395 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Interleukin-36 gamma (Il36g), ELISA Kit

Popular Item
Full Product Name

Mouse Interleukin-36 gamma ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS288173 COA
MBS288173 Testing Data
3D Structure
ModBase 3D Structure for Q8R460
Species Reactivity
Specificity
Recombinant and natural Mouse Interleukin-36 gamma
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
15.6-1000 pg/mL
Intra-assay Precision
Intra-Assay CV: <=6.5%
Inter-assay Precision
Inter-Assay CV: <=9.1%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Il36g elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIl36gpurchase
MBS288173 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-36 gamma (Il36g) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Il36g. The ELISA analytical biochemical technique of the MBS288173 kit is based on Il36g antibody-Il36g antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Il36g antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Il36g. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Il36g elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Il36g elisa kit
Il36g elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS288173. Click to request current manual
NCBI/Uniprot data below describe general gene information for Il36g. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
18,733 Da
NCBI Official Full Name
Interleukin-36 gamma
UniProt Protein Name
Interleukin-36 gamma
UniProt Synonym Protein Names
Interleukin-1 family member 9; IL-1F9
Protein Family
UniProt Gene Name
Il36g  [Similar Products]
UniProt Synonym Gene Names
Il1f9; IL-1F9  [Similar Products]
UniProt Entry Name
IL36G_MOUSE
UniProt Comments for Il36g
Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4+ T-cells and splenocytes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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