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IL4 elisa kit :: Rabbit interleukin 4 ELISA Kit

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Catalog # MBS701120
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $380 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,300 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,340 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

interleukin 4 (IL4), ELISA Kit

Popular Item
Full Product Name

rabbit Interleukin 4, IL-4 ELISA Kit

Product Synonym Names
rabbit Interleukin 4; IL-4 ELISA Kit; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; interleukin 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS701120 Testing Data
3D Structure
ModBase 3D Structure for Q9MZR8
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rabbit IL4. No significant cross-reactivity or interference between Rabbit IL4 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.625 pg/ml-40 pg/ml
Sensitivity
0.156 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Immunity
Protein Biological Process 3
B-cell activation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL4purchase
MBS701120 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interleukin 4 (IL4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL4. The ELISA analytical biochemical technique of the MBS701120 kit is based on IL4 antibody-IL4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL4 elisa kit
For Samples: For the quantitative determination of rabbit interleukin 4 (IL-4) concentrations in serum, plasma, tissue homogenates.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of IL4 elisa kit
IL4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701120. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,562 Da
NCBI Official Full Name
interleukin-4 isoform 1
NCBI Official Symbol
NCBI Official Synonym Symbols
IL2; BSF-1
  [Similar Products]
NCBI Protein Information
interleukin-4; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
UniProt Protein Name
Interleukin-4
UniProt Synonym Protein Names
B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IL-4; BSF-1  [Similar Products]
UniProt Entry Name
IL4_RABIT
UniProt Comments for IL4
Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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