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IL5 elisa kit :: Mouse IL-5 ELISA Kit

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Catalog # MBS175807
Unit / Price
  96-Strip-Wells  /  $380 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

IL-5 (IL5), ELISA Kit

Popular Item
Also Known As

Mouse IL-5 PicoKine ELISA Kit

Product Synonym Names
Interleukin-5; interleukin 5; B cell differentiation factor I; B-cell differentiation factor I; Colony stimulating factor eosinophil; EDF; Eosinophil differentiation factor; IL 5; IL-5; IL5; IL5_HUMAN; Interleukin 5; Interleukin 5 (colony stimulating factor eosinophil); Interleukin-5; Interleukin5; T cell replacing factor; T-cell replacing factor; TRF
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
133
3D Structure
ModBase 3D Structure for P04401
Species Reactivity
Specificity
Natural and recombinant mouse IL-5
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 2pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL5purchase
MBS175807 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-5 (IL5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL5. The ELISA analytical biochemical technique of the MBS175807 kit is based on IL5 antibody-IL5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL5 elisa kit
Principle of the assay: mouse IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-5 has been precoated onto 96-well plates. Standards(sf21,M21-G133) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-5 amount of sample captured in plate.
Background: Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation.1 The predicted amino acid sequence of 134 amino acids is identical with thatrecently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators.2 IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils andeosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3Human and mouse of IL-5 share 70% amino acid sequence homology.

Typical Testing Data/Standard Curve (for reference only) of IL5 elisa kit
Mouse IL-5 PicoKine ELISA Kit standard curve
IL5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175807. Click to request current manual
NCBI/Uniprot data below describe general gene information for IL5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
15,410 Da
NCBI Official Full Name
Interleukin-5
NCBI Official Synonym Full Names
interleukin 5
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
interleukin-5; TRF; BCGF-II; B-cell growth factor II; T-cell replacing factor; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor
UniProt Protein Name
Interleukin-5
UniProt Synonym Protein Names
B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Il-5; IL-5; BCGF-II; TRF  [Similar Products]
UniProt Entry Name
IL5_MOUSE
UniProt Comments for IL5
IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; positive regulation of eosinophil differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with IL5 elisa kit
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