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IL5 elisa kit :: Rabbit Interleukin-5 ELISA Kit

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Catalog # MBS284607
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $745 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,405 +4 FREE 8GB USB
  10x96-Strip-Wells  /  $6,425 +8 FREE 8GB USB
IL5 elisa kit
Product Name

Interleukin-5 (IL5), ELISA Kit

Popular Item
Also Known As

Rabbit Interleukin-5 (IL5) ELISA Kit

Product Synonym Names
EDF; IL-5; TRF; B cell differentiation factor I|T-cell replacing factor|eosinophil differentiation factor|interleukin 5|interleukin-5
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
134
OMIM
147850
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rabbit IL5. No significant cross-reactivity or interference between Rabbit IL5 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIL5purchase
MBS284607 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-5 (IL5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL5. The ELISA analytical biochemical technique of the MBS284607 kit is based on IL5 antibody-IL5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL5 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL5 in samples. An antibody specific for IL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for IL5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,238 Da
NCBI Official Full Name
interleukin-5
NCBI Official Synonym Full Names
interleukin 5
NCBI Official Symbol
NCBI Official Synonym Symbols
EDF; TRF; IL-5
  [Similar Products]
NCBI Protein Information
interleukin-5
UniProt Protein Name
Interleukin-5
UniProt Synonym Protein Names
B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IL-5; TRF  [Similar Products]
UniProt Entry Name
IL5_HUMAN
NCBI Summary for IL5
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
UniProt Comments for IL5
IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding

Biological Process: activation of MAPKK activity; axon guidance; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; inflammatory response; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; positive regulation of immunoglobulin secretion; positive regulation of JAK-STAT cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription factor activity; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with IL5 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >9 publications with IL5 and Thymus
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