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IL7 recombinant protein :: IL-7 Recombinant Protein

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Catalog # MBS232250
Unit / Price
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  0.01 mg  /  $440 +1 FREE 8GB USB
IL7 recombinant protein
Product Name

IL-7 (IL7), Recombinant Protein

Full Product Name

RECOMBINANT HUMAN INTERLEUKIN-7

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
146660
3D Structure
ModBase 3D Structure for P13232
Purity/Purification
>98 % by SDS PAGE and HPLC
Form/Format
Purified recombinant protein expressed in E Coli - lyophilised
Concentration
Total protein concentration 0.1 mg/ml after reconstitution (lot specific)
Reconstitution
Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Buffer Solution
10mM Acetic Acid
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
Other Notes
Small volumes of IL7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IL7 recombinant protein
Recombinant human IL-7 is 17.4 kDa protein comprised of 153 amino acids corresponing to the ful-length mature sequence. IL-7 is a potent lymphoid cell growth factor acting on Pre-B, Pro-B and early T cells. In a dose dependent cell proliferation assay using 2E8 cells this product shows a specific activity of > 2 x 106 units/mg. Recombinant human IL-7 is recognized by Rat anti Human IL-7 antibody clones BVD10-40F6 and BVD10-11C10 in ELISA.
Applications Tested/Suitable for IL7 recombinant protein
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes for IL7 recombinant protein
ELISA: Recombinant human IL-7 may be used as a standard for ELISA applications with either anti-Human IL-7 (purified) or anti-Human IL-7 (biotin conjugate).
Western Blot: Recombinant human IL-7 may be used as the positive control for western blotting applications with either anti-Human IL-7 (purified) or anti-Human IL-7 (biotin conjugate).
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4 ng/well
Functional Assays: Minimum Dilution: 0.1ng/ml; Maximum Dilution: 10ng/ml
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0 ng/lane
NCBI/Uniprot data below describe general gene information for IL7. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Protein Molecular Weight: 17.4KD (153 amino acids)[Similar Products]
NCBI Official Full Name
interleukin-7 isoform 1
NCBI Official Synonym Full Names
interleukin 7
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
interleukin-7
UniProt Protein Name
Interleukin-7
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
IL7_HUMAN
NCBI Summary for IL7
The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]
UniProt Comments for IL7
IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cytokine; Apoptosis; Cell development/differentiation; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q12-q13

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: positive regulation of organ growth; humoral immune response; organ morphogenesis; T cell lineage commitment; cell-cell signaling; positive regulation of T cell differentiation; regulation of gene expression; homeostasis of number of cells within a tissue; positive regulation of cell proliferation; negative regulation of catalytic activity; positive regulation of B cell proliferation; negative regulation of apoptosis; bone resorption
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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